Chelaghma Widad, Loucif Lotfi, Bendjama Esma, Cherak Zineb, Bendahou Mourad, Rolain Jean-Marc
Laboratoire de Microbiologie Appliquée à l'Agroalimentaire, au Biomédical et à l'Environnement, Département de Biologie, Faculté des Sciences de la Nature et de la Vie et des Sciences de la Terre et de l'Univers, Université Abou Bekr Belkaid, Tlemcen 13000, Algeria.
Laboratoire de Biotechnologie des Molécules Bioactives et de la Physiopathologie Cellulaire (LBMBPC), Faculté des Sciences de la Nature et de la Vie, Université Batna 2, Batna 05000, Algeria.
Antibiotics (Basel). 2022 Jul 22;11(8):988. doi: 10.3390/antibiotics11080988.
The aim of this study was to screen for extended spectrum cephalosporin-, carbapenem- and colistin-resistant Gram-negative bacteria in fresh vegetables in Batna, Algeria. A total of 400 samples of fresh vegetables were collected from different retail stores. Samples were immediately subjected to selective isolation, then the representative colonies were identified using matrix-assisted laser desorption ionisation time-of-flight mass spectrometry (MALDI-TOF-MS). Phenotypic and genotypic analyses were carried out in terms of species identification and relative antibiotic resistance. Transferability of the carbapenemase and -bearing plasmids was verified by conjugation. The clonal relationships of carbapenemase and -positive isolates were studied by multi-locus sequence typing (MLST). Sixty-seven isolates were characterised and were mostly isolated from green leafy vegetables, where the dominant species identified included , , , , and . PCR and sequencing results showed that was the bacterial species presenting the highest antibiotic resistance level in parallel to ( = 16) and ( = 11), which were the most detected genes. Moreover, five isolates carried carbapenemase genes, including the and/or genes. The gene was detected in two isolates. MLST analysis revealed three different sequence types: ST101 ( = 1), ST216 ( = 1) and ST2298 ( = 1). Conjugation assays confirmed the transferability of the and genes. In this study we report, for the first time, the detection of the gene in and isolates and the gene in vegetables. To the best of our knowledge, this is the first report on the detection of genes from vegetables in Algeria.
本研究旨在筛查阿尔及利亚巴特纳新鲜蔬菜中对超广谱头孢菌素、碳青霉烯类和黏菌素耐药的革兰氏阴性菌。从不同零售店共采集了400份新鲜蔬菜样本。样本立即进行选择性分离，然后使用基质辅助激光解吸电离飞行时间质谱（MALDI-TOF-MS）鉴定代表性菌落。在菌种鉴定和相对抗生素耐药性方面进行了表型和基因型分析。通过接合试验验证碳青霉烯酶和携带质粒的可转移性。通过多位点序列分型（MLST）研究碳青霉烯酶阳性分离株的克隆关系。鉴定了67株分离株，大多数分离自绿叶蔬菜，其中鉴定出的优势菌种包括[具体菌种名称未给出]。PCR和测序结果表明，[具体菌种名称未给出]是与[另外两个菌种名称未给出]（分别为16株和11株）平行呈现最高抗生素耐药水平的细菌种类，这两个菌种是检测到最多的基因。此外，5株分离株携带碳青霉烯酶基因，包括[具体基因名称未给出]和/或[具体基因名称未给出]基因。在两株[具体菌种名称未给出]分离株中检测到了[具体基因名称未给出]基因。MLST分析揭示了三种不同的[具体菌种名称未给出]序列类型：ST101（1株）、ST216（1株）和ST2298（1株）。接合试验证实了[具体基因名称未给出]和[具体基因名称未给出]基因的可转移性。在本研究中，我们首次报告在[具体菌种名称未给出]和[具体菌种名称未给出]分离株中检测到[具体基因名称未给出]基因以及在蔬菜中检测到[具体基因名称未给出]基因。据我们所知，这是阿尔及利亚首次关于从蔬菜中检测到[具体基因名称未给出]基因的报告。
