一种基于部花青的双模式光学探针用于肼的检测及其在体外和体内的生物成像应用。
A merocyanine-based dual-mode optical probe for detection of hydrazine and its bioimaging application in vitro and vivo.
作者信息
Guo Xiumei, Li Shanshan, Mu Shuai, Zhang Yintang, Liu Xiaoyan, Zhang Haixia
机构信息
State Key Laboratory of Applied Organic Chemistry, Key Laboratory of Nonferrous Metals Chemistry and Resources Utilization of Gansu Province and College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou, 730000, China.
Henan Key Laboratory of Biomolecular Recognition and Sensing, College of Chemistry and Chemical Engineering, Shangqiu Normal University, Shangqiu, 476000, China.
出版信息
Spectrochim Acta A Mol Biomol Spectrosc. 2020 Feb 5;226:117625. doi: 10.1016/j.saa.2019.117625. Epub 2019 Oct 7.
In this paper, a Merocyanine-based turn-on probe (McyA) has been developed for colorimetric and fluorescent dual-mode detection of NH via an intra-molecular charge transfer (ICT) mechanism. In the presence of NH, the probe shows an obvious chromogenic response and fluorescent enhancement. Based on this feature, the synthesized McyA can be applied to quantify NH concentration from 0.12 to 5.0 μM and 0.5-10 μM with a detection limit of 0.042 μM (1.3 ppb, S/N = 3) and 0.140 μM (4.5 ppb, S/N = 3), respectively. Moreover, McyA has also be successfully employed for imaging analysis of NH distribution in different organs from the NH ingested mice model, revealing the potential application of McyA as a powerful fluorescent sensor for tracking detection and risk assessment of hydrazine in vivo.
本文开发了一种基于部花青的开启型探针（McyA），用于通过分子内电荷转移（ICT）机制对NH进行比色和荧光双模式检测。在NH存在下，该探针表现出明显的显色响应和荧光增强。基于此特性，合成的McyA可用于定量0.12至5.0μM和0.5 - 10μM的NH浓度，检测限分别为0.042μM（1.3 ppb，S/N = 3）和0.140μM（4.5 ppb，S/N = 3）。此外，McyA还成功用于对摄入NH的小鼠模型不同器官中NH分布的成像分析，揭示了McyA作为一种强大的荧光传感器在体内跟踪检测肼和风险评估方面的潜在应用。
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