College of Biological Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan, 250353, China.
College of Energy, Xiamen University, Xiamen, 361102, China.
Bioprocess Biosyst Eng. 2020 Feb;43(2):347-355. doi: 10.1007/s00449-019-02230-6. Epub 2019 Oct 12.
Porphyridium purpureum is a rich source for producing phycoerythrin (PE); however, the PE content is greatly affected by culture conditions. Researchers have aimed to optimize the cultivation of P. purpureum for accumulation of PE. When traditional optimized culture conditions were used to cultivate P. purpureum, high PE contents were not usually achieved. In this study, an induced cultivation pattern was applied to P. purpureum for PE biosynthesis (i.e., an incremental approach by altering temperatures, light intensities, and nitrate concentrations). Results revealed that the induced pattern greatly improved the PE biosynthesis. The optimized PE content of 229 mg/L was achieved on the 12th cultivation day, which was a maximum PE content within one cultivation period and accounted for approximately 3.05% of the dry biomass. The induced cultivation pattern was highly suitable for PE synthesis in P. purpureum, which provided an important reference value to the large-scale production of PE.
紫球藻是一种富含藻红蛋白（PE）的生物，然而，PE 的含量会受到培养条件的极大影响。研究人员一直致力于优化紫球藻的培养条件，以积累更多的 PE。当采用传统的优化培养条件来培养紫球藻时，通常无法达到高的 PE 含量。在这项研究中，采用了一种诱导培养模式来进行 PE 生物合成（即通过改变温度、光照强度和硝酸盐浓度的递增方法）。结果表明，该诱导模式极大地促进了 PE 的生物合成。在第 12 天的培养中，PE 的含量达到了 229mg/L，这是一个培养周期内的最大 PE 含量，占干生物量的约 3.05%。这种诱导培养模式非常适合紫球藻中 PE 的合成，为 PE 的大规模生产提供了重要的参考价值。
