Laboratoire de Biotechnologie Végétale Appliquée à l'Amélioration des Cultures, Faculty of Sciences of Sfax, University of Sfax, Sfax, Tunisia; Université Clermont Auvergne, CNRS, SIGMA Clermont, Institut Pascal, F-63000 Clermont-Ferrand, France.
Laboratory of Bioactive Substances, Biotechnology Center of Borj-Cedria (CBBC), BP-901, 2050 Hammam-Lif, Tunisia.
Int J Biol Macromol. 2018 Dec;120(Pt B):2106-2114. doi: 10.1016/j.ijbiomac.2018.09.037. Epub 2018 Sep 7.
The production of B-phycoerythrin (B-PE) from the red microalga Porphyridium marinum was optimized before to purify it and subsequently study its antioxidant activities. NaNO KHPO and metal traces concentrations of the culture medium, and luminosity parameters were chosen, according to the Plackett-Burman design, as the most influent factors on the B-PE production by P. marinum. The optimization of these factors according to the Box-Behnken plan gave a maximum of B-PE production equal to 40 mg/g dry weight under the following conditions: NaNO = 3.4 g/L; KHPO = 0 g/L; light intensity = 70 μmol photons/m/s and metal solution = 1.5 mL/L. The B-PE also showed an interesting capacity to chelate Fe (IC = 13.91 ± 0.21 μg/mL) and a significant reducing power (OD = 0.485 ± 0.011 at 100 μg/mL). The present study reports the antioxidant potential of purified B-PE from P. marinum that could be potentially used as a source of bioactive protein for a wide range of cosmetic and pharmaceutical applications.
在纯化和研究其抗氧化活性之前，对来自红色微藻 Porphyridium marinum 的 B-藻红蛋白（B-PE）进行了生产优化。根据 Plackett-Burman 设计，选择培养基中的 NaNO₃、KH₂PO₄和微量元素浓度以及光照参数作为影响 P. marinum 产生 B-PE 的最主要因素。根据 Box-Behnken 计划对这些因素进行优化，在以下条件下可使 B-PE 的产量达到最大值，为 40mg/g 干重：NaNO₃=3.4g/L；KH₂PO₄=0g/L；光照强度=70μmol 光子/m/s；金属溶液=1.5mL/L。B-PE 还表现出对 Fe 的螯合能力（IC=13.91±0.21μg/mL）和显著的还原能力（在 100μg/mL 时 OD=0.485±0.011）。本研究报告了来自 P. marinum 的纯化 B-PE 的抗氧化潜力，它可能被用作广泛的化妆品和制药应用中生物活性蛋白的来源。
