Artigas F, Sarrias M J, Martínez E, Gelpí E
Life Sci. 1985 Aug 5;37(5):441-7. doi: 10.1016/0024-3205(85)90406-0.
A new HPLC technique for the analysis of picomolar amounts of serotonin (5HT) in plasma and cerebrospinal fluid (CSF) is described. Bufotenin is used as internal standard. Detection is achieved electrochemically or fluorimetrically. The detection limit can be estimated as 50 pg 5HT/mL of either fluid (0.3 picomolar). The method is used to characterize a non-particulate pool of 5HT which is clearly distinct of the platelet pool. Administration of parachlorophenylalanine (PCPA) 300 mg/kg to rats leads to a 90% reduction in the plasmatic pool whereas platelet 5HT is only slightly decreased (3rd day after PCPA) or even increased (7th day after PCPA). Human concentration (n = 15) of 5HT in plasma is 2.6 +/- 0.9 ng/mL (X +/- S.D.). The application of the method to CSF of neurological patients reveals 5HT concentrations ranging from 93 to 962 pg/mL.
本文描述了一种用于分析血浆和脑脊液（CSF）中皮摩尔量血清素（5HT）的新型高效液相色谱（HPLC）技术。蟾蜍色胺用作内标。检测通过电化学或荧光法实现。两种液体中5HT的检测限估计为50 pg/mL（0.3皮摩尔）。该方法用于表征一个明显不同于血小板池的5HT非颗粒池。给大鼠注射300 mg/kg对氯苯丙氨酸（PCPA）会使血浆池减少90%，而血小板5HT仅略有下降（PCPA注射后第3天）或甚至增加（PCPA注射后第7天）。15名人类受试者血浆中5HT的浓度为2.6±0.9 ng/mL（X±标准差）。该方法应用于神经科患者的脑脊液，结果显示5HT浓度范围为93至962 pg/mL。
