Chauveau J, Fert V, Morel A M, Delaage M A
Immunotech, Marseille, France.
Clin Chem. 1991 Jul;37(7):1178-84.
A new, highly sensitive enzyme immunoassay (EIA) of serotonin (5HT) is described. The assay is based on the competition between N-succinyl-glycinamide-serotonin (N-SGA-5HT, obtained by acylation of the 5HT in the sample to be assayed) and an enzymic tracer, N-succinyl-5HT-acetylcholinesterase, for binding to rabbit polyclonal antibody coated onto the wells of microtiter plates. The antibody is directed against an immunogen obtained by coupling N-succinyl-5HT to glycyl-bovine serum albumin. The EIA permits the accurate measurement of as little serotonin as 0.5 nmol/L (1.8 pg per well) in blood, plasma, serum, cerebrospinal fluid, urine, platelets, and other tissues, with no significant cross-reactivity with other compounds. The results obtained correlate well with those obtained by HPLC after extraction. The assay has the advantage of permitting the measurement of 5HT in up to 500 samples in as little as 3 h.
本文描述了一种新的、高灵敏度的血清素（5-羟色胺，5HT）酶免疫测定法（EIA）。该测定法基于N-琥珀酰-甘氨酰胺-血清素（N-SGA-5HT，通过对被测样品中的5HT进行酰化获得）与酶标记物N-琥珀酰-5HT-乙酰胆碱酯酶竞争结合包被在微量滴定板孔中的兔多克隆抗体。该抗体针对通过将N-琥珀酰-5HT与甘氨酰-牛血清白蛋白偶联获得的免疫原。该EIA能够准确测量血液、血浆、血清、脑脊液、尿液、血小板和其他组织中低至0.5 nmol/L（每孔1.8 pg）的血清素，与其他化合物无明显交叉反应。所获得的结果与提取后通过高效液相色谱法（HPLC）获得的结果相关性良好。该测定法的优点是能够在短短3小时内对多达500个样品进行5HT的测量。
