Yadomae T, Suzuki I, Kumazawa Y, Miyazaki T
Microbiol Immunol. 1979;23(10):997-1008. doi: 10.1111/j.1348-0421.1979.tb00530.x.
A water-soluble mitogen was extracted with hot-water from the fruiting bodies of a fungus, Peziza vesiculosa, collected in the wild. The active substance, named vesiculogen, was able to stimulate selectively murine B cells because mitogenic activity was observed in the spleen cell cultures of congenitally athymic nude mice, but not in the thymus cell cultures. The possibility that the mitogenicity of vesiculogen was due to lipopolysaccharide was denied completely by the following evidence: 1) lipopolysaccharide in vesiculogen was undetectable(less than 0.001% in the Limulus test), 2) vesiculogen was able to stimulate strongly DNA synthesis of spleen cells from C3H/HeJ mice, and 3) the mitogenic activity of vesiculogen was not inhibited by polymyxin B. Vesiculogen increased antigen-nonspecifically the number of direct plaque forming cells to sheep erythrocytes, horse erythrocytes, and trinitrophenylated-horse erythrocytes. This result shows that vesiculogen acts as a polyclonal B cell activator on murine spleen cells.
从野外采集的泡质盘菌子实体中用热水提取出一种水溶性促细胞分裂剂。这种活性物质名为泡质盘菌素，能够选择性地刺激小鼠B细胞，因为在先天性无胸腺裸鼠的脾细胞培养物中观察到了促细胞分裂活性，而在胸腺细胞培养物中未观察到。以下证据完全否定了泡质盘菌素的促细胞分裂活性归因于脂多糖的可能性：1）泡质盘菌素中的脂多糖无法检测到（鲎试剂检测中低于0.001%），2）泡质盘菌素能够强烈刺激C3H/HeJ小鼠脾细胞的DNA合成，3）泡质盘菌素的促细胞分裂活性不受多粘菌素B的抑制。泡质盘菌素非特异性地增加了针对绵羊红细胞、马红细胞和三硝基苯基化马红细胞的直接空斑形成细胞的数量。这一结果表明泡质盘菌素对小鼠脾细胞起多克隆B细胞激活剂的作用。
