Zhejiang Provincial Key Laboratory of Biometrology and Inspection and Quarantine, College of Life Sciences, China Jiliang University, Hangzhou, China.
Insect Sci. 2020 Oct;27(5):883-894. doi: 10.1111/1744-7917.12729. Epub 2019 Nov 13.
The microbiome associated with brown planthopper (BPH) plays an important role in mediating host health and fitness. Characterization of the microbial community and its structure is prerequisite for understanding the intricate symbiotic relationships between microbes and host insect. Here, we investigated the bacterial and fungal communities of BPH at different developmental stages using high-throughput amplicon sequencing. Our results revealed that both the bacterial and fungal communities were diverse and dynamic during BPH development. The bacterial communities were generally richer than fungi in each developmental stage. At 97% similarly, 19 phyla and 278 genera of bacteria were annotated, while five fungal phyla comprising 80 genera were assigned. The highest species richness for the bacterial communities was detected in the nymphal stage. The taxonomic diversity of the fungal communities in female adults was generally at a relatively higher level when compared to other developmental stages. The most dominant phylum of bacteria and fungi at each developmental stage all belonged to Proteobacteria and Ascomycota, respectively. A significantly lower abundance of bacterial genus Acinetobacter was recorded in the egg stage when compared to other developmental stages, while the dominant fungal genus Wallemia was more abundant in the nymph and adult stages than in the egg stage. Additionally, the microbial composition differed between male and female adults, suggesting that the microbial communities in BPH were gender-dependent. Overall, our study enriches our knowledge on the microbial communities associated with BPH and will provide clues to develop potential biocontrol techniques against this rice pest.
与褐飞虱（BPH）相关的微生物组在调节宿主健康和适应能力方面发挥着重要作用。微生物群落及其结构的特征是理解微生物与宿主昆虫之间复杂共生关系的前提。在这里，我们使用高通量扩增子测序研究了不同发育阶段 BPH 的细菌和真菌群落。研究结果表明，在 BPH 发育过程中，细菌和真菌群落都是多样且动态的。在每个发育阶段，细菌群落通常比真菌群落更为丰富。在 97%相似性水平上，注释了 19 个门和 278 个属的细菌，而鉴定了包含 80 个属的 5 个真菌门。在若虫阶段，细菌群落的物种丰富度最高。与其他发育阶段相比，雌性成虫的真菌群落的分类多样性通常处于相对较高的水平。在每个发育阶段，细菌和真菌的主要门都分别属于变形菌门和子囊菌门。与其他发育阶段相比，在卵期记录到细菌属不动杆菌的丰度明显较低，而真菌属 Wallemia 在若虫和成虫期比在卵期更为丰富。此外，雌雄成虫之间的微生物组成存在差异，表明 BPH 中的微生物群落具有性别依赖性。总的来说，本研究丰富了我们对与 BPH 相关的微生物群落的认识，并为开发针对这种水稻害虫的潜在生物防治技术提供了线索。
