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犬出血性肠炎:通过电子显微镜检测病毒颗粒

Canine hemorrhagic enteritis: detection of viral particles by electron microscopy.

作者信息

Burtonboy G, Coignoul F, Delferriere N, Pastoret P P

出版信息

Arch Virol. 1979;61(1-2):1-11. doi: 10.1007/BF01320586.

DOI:10.1007/BF01320586
PMID:316320
Abstract

At necropsy, several dogs which died showing symptoms of hemorrhagic diarrhea, had significant lesions of the mucosa that were found especially in the duodenum and upper part of the small bowel. Study of ultrathin sections from the diseased mucosa revealed particles resembling parvoviruses in altered nuclei of cells of the intestinal crypts. Electron microscopic examination of intestinal contents by negative staining has shown the presence of many viral particles which have a diameter of 24 nm and whose profile is consistent with an icosahedral shape. These virions float at a density of 1.43 g/cm3 in cesium chloride and agglutinate rhesus monkey and swine red blood cells at 4 degrees C. A possible etiological role is discussed. This virus is compared with the minute virus of canines and the Feline Panleukopenia virus.

摘要

尸检时,几只死于出血性腹泻症状的狗,其黏膜有明显病变,尤其在十二指肠和小肠上部。对患病黏膜超薄切片的研究显示,在肠隐窝细胞的细胞核改变处发现了类似细小病毒的颗粒。通过负染色对肠道内容物进行电子显微镜检查,发现存在许多直径为24纳米的病毒颗粒,其形态与二十面体形状一致。这些病毒粒子在氯化铯中的密度为1.43克/立方厘米,并在4摄氏度时凝集恒河猴和猪的红细胞。文中讨论了其可能的病因学作用。将这种病毒与犬微小病毒和猫泛白细胞减少症病毒进行了比较。

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Canine hemorrhagic enteritis: detection of viral particles by electron microscopy.犬出血性肠炎:通过电子显微镜检测病毒颗粒
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[Isolation of three kinds of viruses (hog cholera, adenovirus, picodnavirus) in a strain of hog cholera virus designated as cytopathogenic].在一株被指定为细胞病变性的猪霍乱病毒中分离出三种病毒(猪霍乱病毒、腺病毒、微小DNA病毒)
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