Department of Bacteriology and Virology, School of Medicine, Students Research Committee, Shiraz University of Medical Sciences, Shiraz, Iran.
Biotechnology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
Mol Biotechnol. 2023 Oct;65(10):1704-1714. doi: 10.1007/s12033-022-00650-6. Epub 2023 Feb 9.
Iron coating was introduced as one of the novel techniques to improve physicochemical and biological properties of silver nanoparticles (AgNPs). In the current experiment, impact of iron coating on the antimicrobial potency of AgNPs was investigated against methicillin-resistance Staphylococcus aureus (MRSA). To obtain more accurate data about the antimicrobial potency of examined nanostructures, the experiment was done on the 10 isolates of MRSA which were isolated from skin lesions. AgNPs and iron-coated AgNPs (Fe@AgNPs) were fabricated based on a green one-pot reaction procedure. Minimal inhibitory concentration (MIC) of Fe@AgNPs was not significantly different with MIC of AgNPs against eight out of 10 examined MRSA isolates. Also, by iron coating a reduction in the minimal inhibitory concentration (MIC) of AgNPs was observed against two MRSA isolates. The average MIC of AgNPs against 10 MRSA isolates was calculated to be 2.16 ± 0.382 mg/mL and this value was reduced to 1.70 ± 0.638 mg/mL for Fe@AgNPs. However, this difference was not considered significant statistically (P-value > 0.05). From productivity point of view, it was found that iron coating would improve the productivity of the synthesis reaction more than fivefold. Productivity of AgNPs was calculated to be 1.02 ± 0.07 g/L, meanwhile this value was 5.25 ± 0.05 g/L for Fe@AgNPs. Iron coating may provide another economic benefit to reduce final price of AgNPs. It is obvious that the price of a particular nanostructure made of silver and iron is significantly lower than that of pure silver. These findings can be considered for the fabrication of economic and potent antimicrobial nanoparticles.
铁涂层被引入作为一种提高银纳米粒子（AgNPs）物理化学和生物性质的新技术。在当前的实验中，研究了铁涂层对耐甲氧西林金黄色葡萄球菌（MRSA）的抗菌效力的影响。为了获得关于被检查的纳米结构的抗菌效力的更准确数据，该实验在从皮肤损伤中分离出的 10 株耐甲氧西林金黄色葡萄球菌上进行。AgNPs 和铁涂层的 AgNPs（Fe@AgNPs）是基于绿色一锅反应程序制备的。Fe@AgNPs 的最小抑菌浓度（MIC）与 AgNPs 对 10 株被检查的耐甲氧西林金黄色葡萄球菌分离株中的 8 株的 MIC 没有显著差异。此外，通过铁涂层，AgNPs 的最小抑菌浓度（MIC）对 2 株耐甲氧西林金黄色葡萄球菌分离株降低。AgNPs 对 10 株耐甲氧西林金黄色葡萄球菌分离株的平均 MIC 计算为 2.16±0.382mg/mL，而 Fe@AgNPs 的 MIC 值降低至 1.70±0.638mg/mL。然而，从统计学角度来看，这一差异并不显著（P 值>0.05）。从生产效率的角度来看，发现铁涂层会使合成反应的生产效率提高五倍以上。AgNPs 的生产效率计算为 1.02±0.07g/L，而 Fe@AgNPs 的生产效率为 5.25±0.05g/L。铁涂层可能会提供另一个经济优势，以降低 AgNPs 的最终价格。显然，由银和铁制成的特定纳米结构的价格明显低于纯银的价格。这些发现可用于制备经济有效的抗菌纳米粒子。
