State Key Laboratory of Eco-hydraulic Engineering in Arid Area, Xi'an University of Technology, Xi'an 710048, China.
State Key Laboratory of Eco-hydraulic Engineering in Arid Area, Xi'an University of Technology, Xi'an 710048, China.
Bioresour Technol. 2019 Nov;292:122011. doi: 10.1016/j.biortech.2019.122011. Epub 2019 Aug 14.
This study explored the effects of Bacillus subtilis at four levels (0, 0.5%, 1%, and 2% w/w compost) on the variations in ARGs, mobile genetic elements (MGEs), and the bacterial community during composting. The composting process had a greater impact on ARGs than Bacillus subtilis. The main ARG detected was sul1. The addition of Bacillus subtilis at 0.5% reduced the relative abundances of ARGs, MGEs, and human pathogenic bacteria (by 2-3 logs) in the mature products. Network and redundancy analyses suggested that intI1, Firmicutes, and pH were mainly responsible for the changes in ARGs, thus controlling these factors might help to inhibit the spread of ARGs.
本研究探讨了枯草芽孢杆菌在四个水平（0、0.5%、1%和 2%w/w 堆肥）下对堆肥过程中 ARGs、移动遗传元件（MGEs）和细菌群落变化的影响。堆肥过程对 ARGs 的影响大于枯草芽孢杆菌。主要检测到的 ARG 是 sul1。在成熟产品中，添加 0.5%的枯草芽孢杆菌可降低 ARGs、MGEs 和人致病菌的相对丰度（减少 2-3 个对数）。网络和冗余分析表明，intI1、Firmicutes 和 pH 主要负责 ARGs 的变化，因此控制这些因素可能有助于抑制 ARGs 的传播。
