Department of Analytical Chemistry, Medical University of Lublin, Chodźki 4A, 20-093 Lublin, Poland.
Molecules. 2019 Oct 21;24(20):3790. doi: 10.3390/molecules24203790.
The aim of the study was to estimate the thiocyanate levels in saliva of cigarette smokers in comparison to e-cigarette smokers and nonsmokers. To improve our understanding of the influence of smoking on the oral level of thiocyanate, we conducted an assessment of human saliva, in 24 individuals (eight tobacco smokers, eight e-cigarette smokers, and eight nonsmokers). High-Performance Liquid Chromatography with ultraviolet detection (HPLC-UV) using a unique phosphatidylcholine column was applied in this assay. Thiocyanate ion was detected directly by its absorbance at 210 nm. The method presents a new application of the IAM (Immobilized Artificial Membrane) column for quantification of inorganic anions. The whole process meets the criteria of green chemistry because it was carried out without the use of organic solvents. For compensating matrix effects, an eight-point standard addition protocol was used to quantify the thiocyanate level in saliva samples. The calibration graphs were linear in the range of 5-100 mg L with a correlation coefficient higher than 0.99. The thiocyanate concentrations in the saliva of tobacco smokers, e-cigarette smokers, and nonsmokers were found in the range of 121.25-187.54 mg L, 121.24-244.11 mg L, 33.03-79.49 mg L, respectively. The present study indicates an obvious statistically significant elevation in salivary thiocyanate level in tobacco smokers in comparison to nonsmokers. The phosphatidylcholine-based stationary phase proved to be suitable for the detection and quantification of the thiocyanate ion. The salivary thiocyanate levels in e-cigarette smokers were not significantly different in comparison to tobacco smokers but higher if compared to nonsmokers. The criterion for statistical significance was p < 0.05.
本研究旨在比较吸烟人群、电子烟使用者和不吸烟者的唾液中硫氰酸盐水平。为了更好地了解吸烟对唾液中硫氰酸盐水平的影响，我们对 24 名个体（8 名烟民、8 名电子烟使用者和 8 名非吸烟者）的唾液进行了评估。该检测采用高效液相色谱法（HPLC-UV）结合独特的磷脂酰胆碱柱，直接通过 210nm 处的吸光度检测硫氰酸根离子。该方法为无机阴离子的定量分析提供了一种 IAM（固定化人工膜）柱的新应用。由于整个过程不使用有机溶剂，因此符合绿色化学的标准。为了补偿基质效应，采用八点标准加入法来定量唾液样本中的硫氰酸盐水平。校准曲线在 5-100mg/L 范围内呈线性，相关系数高于 0.99。烟民、电子烟使用者和非吸烟者的唾液中硫氰酸盐浓度分别在 121.25-187.54mg/L、121.24-244.11mg/L 和 33.03-79.49mg/L 范围内。本研究表明，与非吸烟者相比，烟民的唾液中硫氰酸盐水平明显升高，具有统计学显著意义。基于磷脂酰胆碱的固定相被证明适合于硫氰酸根离子的检测和定量。与烟民相比，电子烟使用者的唾液中硫氰酸盐水平没有显著差异，但与非吸烟者相比则更高。统计学显著水平的标准为 p<0.05。
