Tanaka J, Fujiwara H, Torisu M
Immunology. 1979 Dec;38(4):727-34.
The effects of vitamin E on the humoral immune response to hamster erythrocytes (HRBC) and 2,4,6-trinitrophenyl (TNP) were studied in mice. Inbred SL mice were fed on a diet supplemented with 0, 20 or 200 mg of vitamin E per kg of food throughout the course of experiments. These mice were immunized primarily with HRBC 50 days after the beginning of treatment with vitamin E supplementation. Secondary immunization with TNP—HRBC, a hapten-carrier conjugate, was given 28 days after primary immunization with HRBC. Anti-HRBC and anti-TNP haemagglutinin titres were increased by supplementing mice with vitamin E. Moreover, the effect of previous priming of mice with HRBC on the hapten-specific antibody response to immunization with TNP-HRBC was also enhanced by vitamin E supplementation. These effects of vitamin E were dose-dependent, and vitamin E as tocopheryl acetate exerted more effect than vitamin E as tocopheryl nicotinate. In experiments with the mouse inbred strain DDD, vitamin E seemed to facilitate the shift of antibody production from IgM to IgG. Initial IgM response and late IgG response were not augmented by treating mice with vitamin E supplementation. These findings suggested that vitamin E stimulated the helper activity of T lymphocytes. This was confirmed using an adoptive transfer system involving stimulation of hapten-primed cells by a hapten-carrier conjugate in the presence of carrier-primed helper cells.
在小鼠中研究了维生素E对仓鼠红细胞（HRBC）和2,4,6-三硝基苯（TNP）体液免疫反应的影响。在整个实验过程中，将近交系SL小鼠饲养在每千克食物补充0、20或200毫克维生素E的饮食中。在用维生素E补充剂治疗开始50天后，用HRBC对这些小鼠进行初次免疫。在用HRBC初次免疫28天后，用半抗原-载体结合物TNP-HRBC进行二次免疫。给小鼠补充维生素E可提高抗HRBC和抗TNP血凝素滴度。此外，预先用HRBC对小鼠进行致敏对用TNP-HRBC免疫的半抗原特异性抗体反应的影响也因补充维生素E而增强。维生素E的这些作用呈剂量依赖性，作为醋酸生育酚的维生素E比作为烟酸生育酚的维生素E发挥的作用更大。在对近交系DDD小鼠进行的实验中，维生素E似乎促进了抗体产生从IgM向IgG的转变。用维生素E补充剂处理小鼠并没有增强最初的IgM反应和后期的IgG反应。这些发现表明维生素E刺激了T淋巴细胞的辅助活性。使用涉及在载体致敏的辅助细胞存在下用半抗原-载体结合物刺激半抗原致敏细胞的过继转移系统证实了这一点。
