Kettman J, Dutton R W
Proc Natl Acad Sci U S A. 1971 Apr;68(4):699-703. doi: 10.1073/pnas.68.4.699.
The in vitro primary response of mouse spleen cell suspensions to 2,4,6-trinitrophenyl(Tnp)-erythrocytes has been studied. The number of anti-Tnp plaque-forming cells that arise after antigenic stimulation in vitro is greatly enhanced by prior immunization in vivo with the carrier erythrocyte. The enhancement is antigen specific. The priming for an enhanced response can be elicited with very low antigen doses and is often apparent 24 hr after immunization. It is marked from day 3 to day 14. Spleen cells from carrier-primed mice will enhance the anti-Tnp response of normal cells when mixed cultures of the two cell populations are challenged with Tnp-erythrocytes in vitro. The carrier-primed cells mediating this enhancing effect are thymus derived. The development of the thymus-derived, carrier-specific cell population has been generally assumed to involve the antigenic stimulation of cell proliferation. It was, therefore, somewhat surprising to find that the enhancing effect of the carrier-primed cells, once they had been generated, is not inhibited by x-irradiation.
对小鼠脾细胞悬液针对2,4,6-三硝基苯（Tnp）-红细胞的体外初次反应进行了研究。体外抗原刺激后产生的抗Tnp噬斑形成细胞的数量，通过事先用载体红细胞进行体内免疫而大大增加。这种增强是抗原特异性的。用非常低的抗原剂量就能引发增强反应的致敏，且在免疫后24小时通常就很明显。在第3天到第14天很显著。当用Tnp-红细胞在体外对这两种细胞群体的混合培养物进行攻击时，来自载体致敏小鼠的脾细胞会增强正常细胞的抗Tnp反应。介导这种增强作用的载体致敏细胞是胸腺来源的。一般认为胸腺来源的、载体特异性细胞群体的发育涉及细胞增殖的抗原刺激。因此，发现一旦载体致敏细胞产生后，其增强作用不受X射线照射的抑制，这有点令人惊讶。
