Kitisripanya Tharita, Sermpradit Witcha, Sakamoto Seiichi, Tanaka Hiroyuki, Putalun Waraporn
Department of Pharmacognosy, Faculty of Pharmacy, Mahidol University, Bangkok, Thailand.
Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen, Thailand.
Biomed Chromatogr. 2020 Feb;34(2):e4718. doi: 10.1002/bmc.4718. Epub 2019 Dec 18.
There have been reports of fake artesunate (ART), which has led to deaths from untreated malaria in South East Asia. To rapidly screen for fake and adulterated ART products in the drug market, a lateral flow immunoassay (LFIA) based on a colloidal gold-monoclonal antibody probe for detection of ART within samples was developed. With this method, the calibration curve for ART was determined by the intensity ratio of the test and control bands at various ART concentrations. The linearity range was 12.5-200 μg/ml of ART. Samples were tested by the developed LFIA and can be calculated for ART contents. The levels of ART in the samples were also confirmed by enzyme-linked immunosorbent assay. The results of the two methods were in good conformance. The proposed LFIA was demonstrated to be a simple and rapid analytical method for detecting ART in the pharmaceutical formulation.
有报告称存在假冒青蒿琥酯（ART）的情况，这已导致东南亚地区因疟疾未得到治疗而死亡。为了快速筛查药品市场上假冒和掺假的ART产品，开发了一种基于胶体金 - 单克隆抗体探针的侧向流动免疫分析（LFIA）方法，用于检测样品中的ART。采用这种方法，通过在不同ART浓度下测试带和对照带的强度比来确定ART的校准曲线。线性范围为12.5 - 200μg/ml的ART。用所开发的LFIA对样品进行检测，并可计算出ART含量。样品中的ART水平也通过酶联免疫吸附测定法进行了确认。两种方法的结果具有良好的一致性。所提出的LFIA被证明是一种用于检测药物制剂中ART的简单快速的分析方法。
