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利用偏振二次谐波产生显微镜揭示人类心脏组织中胶原蛋白的极性组织。

Polar organization of collagen in human cardiac tissue revealed with polarimetric second-harmonic generation microscopy.

作者信息

Mirsanaye Kamdin, Golaraei Ahmad, Habach Fayez, Žurauskas Edvardas, Venius Jonas, Rotomskis Ricardas, Barzda Virginijus

机构信息

Department of Physics, University of Toronto, 60 St. George St, Toronto, M5S 1A7, Canada.

Department of Chemical and Physical Sciences, University of Toronto Mississauga, 3359 Mississauga Rd North, Mississauga, L5L 1C6, Canada.

出版信息

Biomed Opt Express. 2019 Sep 10;10(10):5025-5030. doi: 10.1364/BOE.10.005025. eCollection 2019 Oct 1.

DOI:10.1364/BOE.10.005025
PMID:31646027
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6788612/
Abstract

Polarimetric second-harmonic generation (P-SHG) microscopy is used to characterize the composition and polarity of collagen fibers in various regions of human cardiac tissue. The boundary between the cardiac conduction system and myocardium is shown to possess a distinct composition of collagen compared to other regions in the heart. Moreover, collagen fibers in this region are macroscopically organized in a unipolar arrangement, which may consequently aid in effective propagation of the electrical signal through the cardiac conduction system.

摘要

偏振二次谐波产生(P-SHG)显微镜用于表征人类心脏组织不同区域中胶原纤维的组成和极性。与心脏的其他区域相比,心脏传导系统与心肌之间的边界显示出具有独特的胶原组成。此外,该区域的胶原纤维在宏观上呈单极排列,这可能有助于电信号通过心脏传导系统的有效传播。

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Collagen chirality and three-dimensional orientation studied with polarimetric second-harmonic generation microscopy.用偏光二次谐波产生显微镜研究胶原蛋白的手性和三维取向。
J Biophotonics. 2019 Jan;12(1):e201800241. doi: 10.1002/jbio.201800241. Epub 2018 Oct 14.
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