Kousi Maria, Kalogiouri Natasa P, Samanidou Victoria F
Laboratory of Analytical Chemistry, School of Chemistry, Aristotle University of Thessaloniki, Thessaloniki, Greece.
J Sep Sci. 2025 Feb;48(2):e70096. doi: 10.1002/jssc.70096.
This review highlights recent advances in the bioanalysis of cephalosporins using liquid chromatographic methods, focusing on green sample preparation (GSP) techniques. Cephalosporins, a class of β-lactam antibiotics, are critical in combating bacterial infections but present challenges related to drug resistance and toxicity. This article evaluates various sample preparation methods, including solid-phase extraction, solid-phase microextraction, and protein precipitation, which have been employed in the extraction and quantification of cephalosporins from biological matrices. Special attention is given to the optimization of critical parameters, such as pH, extraction solvents, and purification techniques to maximize analytes' recovery and sensitivity. Emerging trends in GSP, such as the use of molecularly imprinted polymers and miniaturized processing devices, are also discussed. The review underscores the growing importance of integrating environmentally friendly approaches in cephalosporin bioanalysis, paving the way for future innovations in bioanalytical research.
本综述重点介绍了使用液相色谱法进行头孢菌素生物分析的最新进展，重点关注绿色样品制备（GSP）技术。头孢菌素是一类β-内酰胺抗生素，在对抗细菌感染方面至关重要，但存在与耐药性和毒性相关的挑战。本文评估了各种样品制备方法，包括固相萃取、固相微萃取和蛋白质沉淀，这些方法已用于从生物基质中提取和定量头孢菌素。特别关注关键参数的优化，如pH值、萃取溶剂和纯化技术，以最大限度地提高分析物的回收率和灵敏度。还讨论了GSP的新兴趋势，如分子印迹聚合物的使用和小型化处理设备。该综述强调了在头孢菌素生物分析中整合环境友好方法的重要性日益增加，为生物分析研究的未来创新铺平了道路。
