静脉曲张患者分化的M2巨噬细胞中miR-661、miR-1202、lncRNA-HOTAIR、lncRNA-GAS5与基质金属蛋白酶9之间的关联

Associations between miR-661, miR-1202, lncRNA-HOTAIR, lncRNA-GAS5 and MMP9 in differentiated M2-macrophages of patients with varicose veins.

作者信息

Biranvand Azin S, Khosravi Mohsen, Esfandiari Golnaz, Poursaleh Adeleh, Hosseini-Fard Sayed R, Amirfarhangi Abdollah, Najafi Mohammad

机构信息

Cellular and Molecular Research Center, Biochemistry Department, Iran University of Medical Sciences, Tehran, Iran.

Hazrat-e Rasool Hospital, Iran University of Medical Sciences, Tehran, Iran.

出版信息

Int Angiol. 2018 Dec;37(6):451-456. doi: 10.23736/S0392-9590.18.04022-1.

DOI:10.23736/S0392-9590.18.04022-1

PMID:30558403

Abstract

BACKGROUND

The venous hypertension is suggested as the main cause of varicose disease. Some mediators and growth factors are known as the responsible of cellular events for the progression of venous perturbations. The aim of this study was to investigate non-coding (nc) RNA and MMP9 expression levels in macrophages differentiated from monocytes of patients with varicose veins.

METHODS

The monocytes were isolated from the whole blood samples by RosetteSep kit and were differentiated to macrophages M2 using M-CSF factor. The based on ncRNA-gene network, lncRNA-GAS5, lncRNA-HOTAIR, miRNA-661, miRNA-1202, and MMP9 were selected. The gene expression levels were measured by RT-qPCR technique.

RESULTS

Data showed that the MMP9 gene expression increased (P=0.003) while the GAS5, miRNA-661, and miRNA-1202 expression levels reduced significantly in the differentiated macrophages of patients (P=0.035, P=0.009, and P=0.015, respectively). Furthermore, the MMP9 gene expression levels were conversely related to the GAS5, HOTAIR, miRNA-661 and miRNA-1202 expression levels.

CONCLUSIONS

The results suggested that the lncRNA-GAS5, miRNA-661, miRNA-1202 and MMP9 are involved in varicose disease.

摘要

背景

静脉高压被认为是静脉曲张疾病的主要原因。一些介质和生长因子被认为是导致静脉病变进展的细胞事件的原因。本研究的目的是调查静脉曲张患者单核细胞分化而来的巨噬细胞中非编码（nc）RNA和MMP9的表达水平。

方法

通过RosetteSep试剂盒从全血样本中分离单核细胞，并使用M-CSF因子将其分化为M2巨噬细胞。基于ncRNA-基因网络，选择lncRNA-GAS5、lncRNA-HOTAIR、miRNA-661、miRNA-1202和MMP9。通过RT-qPCR技术测量基因表达水平。

结果

数据显示，患者分化的巨噬细胞中MMP9基因表达增加（P=0.003），而GAS5、miRNA-661和miRNA-1202的表达水平显著降低（分别为P=0.035、P=0.009和P=0.015）。此外，MMP9基因表达水平与GAS5、HOTAIR、miRNA-661和miRNA-1202的表达水平呈负相关。