State Key Laboratory of Microbial Technology, Marine Biotechnology Research Center, Shandong University, Qingdao, 266237, China.
Laboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao, 266237, China.
Antonie Van Leeuwenhoek. 2020 Mar;113(3):357-364. doi: 10.1007/s10482-019-01346-9. Epub 2019 Oct 28.
A Gram-stain negative, aerobic, non-flagellated, rod-shaped bacterium, designated strain SM1810, was isolated from deep-sea sediment collected from the Southwest Indian Ocean. Strain SM1810 grows at 15-40 °C (optimum, 28 °C), at pH 5.0-9.0 (optimum, pH 6.0-7.0) and with 0-8% (w/v) NaCl (optimum, 1.5%). Phylogenetic analysis of 16S rRNA gene sequences revealed that strain SM1810 is affiliated with the genus Pedobacter, sharing high sequence similarity (95.8%) with the type strain of Pedobacter bauzanensis. The major fatty acids of strain SM1810 are iso-C, summed feature 3 (Cω7c and/or Cω6c) and iso-C 3OH. The predominant respiratory quinone is MK-7. The polar lipids are phosphatidylethanolamine, an unidentified aminophospholipid, an unidentified phospholipid and eight unidentified lipids. The genomic DNA G + C content of strain SM1810 was determined to be 40.8 mol%. Based on the phylogenetic, chemotaxonomic and phenotypic data obtained in this study, strain SM1810 is concluded to represent a novel species of the genus Pedobacter, for which the name Pedobacter indicus sp. nov. is proposed. The type strain is SM1810 (KCTC 62798 = CCTCC AB 2018198).
一株革兰氏阴性、需氧、无鞭毛、杆状细菌，被命名为 SM1810 菌株，从西南印度洋深海沉积物中分离得到。SM1810 菌株在 15-40°C（最佳温度 28°C）、pH5.0-9.0（最佳 pH6.0-7.0）和 0-8%（w/v）NaCl（最佳 1.5%）的条件下生长。16S rRNA 基因序列的系统发育分析表明，SM1810 菌株属于 Pedobacter 属，与 Pedobacter bauzanensis 模式菌株的序列相似性高达 95.8%。SM1810 菌株的主要脂肪酸为 iso-C、总和特征 3（Cω7c 和/或 Cω6c）和 iso-C 3OH。主要呼吸醌为 MK-7。极性脂为磷脂酰乙醇胺、一种未鉴定的氨基磷脂、一种未鉴定的磷脂和八种未鉴定的脂类。SM1810 菌株的基因组 DNA G+C 含量为 40.8mol%。基于本研究中获得的系统发育、化学分类和表型数据，SM1810 菌株被认为代表了 Pedobacter 属的一个新种，命名为印度 Pedobacter 新种。模式菌株为 SM1810（KCTC 62798=CCTCC AB 2018198）。
