School of Chemical Engineering , Sichuan University , Chengdu 610065 , China.
ACS Appl Mater Interfaces. 2019 Nov 27;11(47):43835-43842. doi: 10.1021/acsami.9b13614. Epub 2019 Nov 12.
A novel self-assembling peptide-functionalized core-shell mesoporous silica nanoparticle was developed as a drug carrier. Superparamagnetic manganese- and cobalt-doped iron oxide nanoparticles formed the core for the mesoporous silica shell coating. On the silica outer shell, the peptide Boc-Phe-Phe-Gly-Gly-COOH was covalently conjugated by 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride and -hydroxysulfosuccinimide sodium salt coupling. The self-assembling property of the peptide at physiological temperature was utilized to block the pore openings, while the disassembly at elevated local particle temperature released cargo molecules without bulk heating that would cause cell damage. Both conventional heating and heating in an alternating magnetic field were tested for the release of fluorescein and daunorubicin. In vitro experiments showed high cytotoxicity on pancreatic carcinoma cells (PANC-1) when this delivery system was activated by an alternating magnetic field, while control particles without drugs showed no obvious cytotoxicity.
一种新型的自组装肽功能化核壳介孔硅纳米颗粒被开发为药物载体。超顺磁性锰和钴掺杂的氧化铁纳米颗粒形成了介孔硅壳涂层的核。在硅外壳上，通过 1-乙基-3-(3-二甲基氨基丙基)碳化二亚胺盐酸盐和 -羟基磺基琥珀酰亚胺钠盐偶联将 Boc-Phe-Phe-Gly-Gly-COOH 肽共价连接。利用肽在生理温度下的自组装特性来封闭孔口，而在升高的局部颗粒温度下解组装会释放货物分子，而不会引起会导致细胞损伤的整体加热。对荧光素和柔红霉素的释放进行了常规加热和交变磁场加热的测试。体外实验表明，当该递药系统通过交变磁场激活时，对胰腺癌细胞（PANC-1）具有高细胞毒性，而没有药物的对照颗粒则没有明显的细胞毒性。
