Miao Zhonghua, Cheng Ruyue, Zhang Yujie, Liang Huijing, Jiang Fengling, Shen Xi, Chen Gong, Zhang Qisheng, He Fang, Li Ming
Department of Nutrition, Food Hygiene and Toxicology, West China School of Public Health and West China Fourth Hospital, and Healthy Food Evaluation Research Center, Sichuan University, Chengdu, Sichuan, PR China.
Sichuan Acadmey of Food and Fermentation Industries, Chengdu, Sichuan, PR China.
Biosci Biotechnol Biochem. 2020 Feb;84(2):411-420. doi: 10.1080/09168451.2019.1676696. Epub 2019 Oct 29.
This study assessed whether antibiotics could alter gut microbiota to affect host growth and the possibility of alleviation by lactobacilli. We divided four-week-old BABL/c mice into control (Ctrl), antibiotic exposure (Abx), Lactobacillus plantarum PC-170 (PC), and Lactobacillus rhamnosus GG (LGG) group and the Abx, LGG, and PC group received an one-week antibiotic/antibiotic + probiotic treatment. The fecal microbiota and the expression of splenic cytokines were determined. Following the ceftriaxone treatment, the body weight gain of Abx was delayed compared with others. The ceftriaxone treatment significantly decreased the alpha-diversity of the fecal microbiota and altered the fecal microbiota but LGG and PC can partly alleviate the effect. At the end of the study, the microbial community of LGG and PC group were more similar to Ctrl compared with Abx group. The results indicated that ceftriaxone could significantly alter intestinal microbiota. Lactobacilli might alleviate the side effects of antibiotics by stabilizing the intestinal microbiota.
本研究评估了抗生素是否会改变肠道微生物群以影响宿主生长，以及乳酸菌缓解这种影响的可能性。我们将四周龄的BABL/c小鼠分为对照组（Ctrl）、抗生素暴露组（Abx）、植物乳杆菌PC-170（PC）组和鼠李糖乳杆菌GG（LGG）组，Abx、LGG和PC组接受为期一周的抗生素/抗生素+益生菌治疗。测定了粪便微生物群和脾脏细胞因子的表达。头孢曲松治疗后，Abx组的体重增加比其他组延迟。头孢曲松治疗显著降低了粪便微生物群的α多样性并改变了粪便微生物群，但LGG和PC可以部分缓解这种影响。在研究结束时，与Abx组相比，LGG和PC组的微生物群落与Ctrl组更相似。结果表明，头孢曲松可显著改变肠道微生物群。乳酸菌可能通过稳定肠道微生物群来减轻抗生素的副作用。
