Research group on Advance Materials and Energy MatyEr, Biomaterials and Electromedicine Laboratory, Instituto Tecnológico Metropolitano, Calle 54A No. 30-01, Medellín 050013, Colombia.
Research group on Automatic, Electronic and Computational Science, MIRP Laboratory, Instituto Tecnológico Metropolitano, Calle 54A No. 30-01, Medellín 050013, Colombia.
Sensors (Basel). 2019 Oct 28;19(21):4674. doi: 10.3390/s19214674.
Cutaneous leishmaniasis (CL) is a neglected tropical disease that requires novel tools for its understanding, diagnosis, and treatment follow-up. In the cases of other cutaneous pathologies, such as cancer or cutaneous ulcers due to diabetes, optical diffuse reflectance-based tools and methods are widely used for the investigation of those illnesses. These types of tools and methods offer the possibility to develop portable diagnosis and treatment follow-up systems. In this article, we propose the use of a three-layer diffuse reflectance model for the study of the formation of cutaneous ulcers caused by CL. The proposed model together with an inverse-modeling procedure were used in the evaluation of diffuse-reflectance spectral signatures acquired from cutaneous ulcers formed in the dorsal area of 21 golden hamsters inoculated with Leishmanisis braziliensis. As result, the quantification of the model's variables related to the main biological parameters of skin were obtained, such as: diameter and volumetric fraction of keratinocytes, collagen; volumetric fraction of hemoglobin, and oxygen saturation. Those parameters show statistically significant differences among the different stages of the CL ulcer formation. We found that these differences are coherent with histopathological manifestations reported in the literature for the main phases of CL formation.
皮肤利什曼病（CL）是一种被忽视的热带病，需要新的工具来了解、诊断和跟踪治疗。在其他皮肤疾病的情况下，如癌症或由于糖尿病引起的皮肤溃疡，基于光学漫反射的工具和方法被广泛用于这些疾病的研究。这些类型的工具和方法提供了开发便携式诊断和治疗随访系统的可能性。在本文中，我们提出使用三层漫反射模型来研究由 CL 引起的皮肤溃疡的形成。所提出的模型与逆模型程序一起用于评估从 21 只接种巴西利什曼原虫的金黄地鼠背部区域形成的皮肤溃疡获得的漫反射光谱特征。结果，获得了与皮肤主要生物学参数相关的模型变量的定量，例如：角朊细胞、胶原蛋白的直径和体积分数；血红蛋白和氧饱和度的体积分数。这些参数在 CL 溃疡形成的不同阶段显示出统计学上的显著差异。我们发现这些差异与文献中报道的 CL 形成的主要阶段的组织病理学表现一致。
