Hefei National Laboratory for Physical Sciences at the Microscale, CAS Key Laboratory of Brain Function and Disease, School of Life Sciences, University of Science and Technology of China, Hefei, Anhui 230027, People's Republic of China.
CAS Key Laboratory of Mechanical Behavior and Design of Materials, Hefei National Laboratory for Physical Science at the Microscale, Department of Modern Mechanics, CAS Center for Excellence in Complex System Mechanics, University of Science and Technology of China, Hefei, Anhui 230027, People's Republic of China.
Open Biol. 2019 Oct 31;9(10):190137. doi: 10.1098/rsob.190137. Epub 2019 Oct 30.
One of the major topics in magnetobiology is the biological effects of strong static magnetic field (SMF) on living organisms. However, there has been a paucity of the comprehensive study of the long-term effects of strong SMF on an animal's development. Here, we explored this question with zebrafish, an excellent model organism for developmental study. In our research, zebrafish eggs, just after fertilization, were exposed to a 9.0 T SMF for 24 h, the critical period of post-fertilization development from cleavage to segmentation. The effects of strong SMF exposure on the following developmental progress of zebrafish were studied until 6 days post-fertilization (dpf). Results showed that 9.0 T SMF exposure did not influence the survival or the general developmental scenario of zebrafish embryos. However, it slowed down the developmental pace of the whole animal, and the late developers would catch up with their control peers after the SMF was removed. We proposed a mechanical model and deduced that the development delaying effect was caused by the interference of SMF in microtubule and spindle positioning during mitosis, especially in early cleavages. Our research data provide insights into how strong SMF influences the developing organisms through basic physical interactions with intracellular macromolecules.
磁生物学的主要课题之一是强静磁场（SMF）对生物体的生物学效应。然而，对于强 SMF 对动物发育的长期影响，缺乏全面的研究。在这里，我们用斑马鱼来探讨这个问题，斑马鱼是发育研究的优秀模式生物。在我们的研究中，斑马鱼卵在受精后立即暴露于 9.0 T 的 SMF 中 24 小时，这是从分裂到分节的受精后发育的关键时期。研究了强 SMF 暴露对受精后 6 天（dpf）的斑马鱼以下发育进程的影响。结果表明，9.0 T SMF 暴露不会影响斑马鱼胚胎的存活率或一般发育情况。然而，它减缓了整个动物的发育速度，并且在 SMF 去除后，晚期发育者会赶上其对照同伴。我们提出了一个力学模型，并推断这种发育延迟效应是由于 SMF 在有丝分裂过程中对微管和纺锤体定位的干扰引起的，尤其是在早期分裂中。我们的研究数据提供了关于强 SMF 通过与细胞内大分子的基本物理相互作用如何影响发育中的生物体的见解。
