Cheng Lei, Wang Mudi, Yang Baolin, Li Yang, Wang Tong, Xi Chuanying, Han Yuyan, Wang Ze, Fang Yanwen, Wei Min, Du Hua, Xu An
Key Laboratory of High Magnetic Field and Ion Beam Physical Biology, Anhui Province Key Laboratory of Environmental Toxicology and Pollution Control Technology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, Anhui 230031, PR China; School of Environmental Science and Optoelectronic Technology, University of Science and Technology of China, Hefei, Anhui 230026, PR China.
School of Basic Medical Science, Anhui Medical University, Hefei, Anhui 230032, PR China.
J Adv Res. 2024 Jul 31. doi: 10.1016/j.jare.2024.07.032.
Ultra-high static magnetic fields (SMFs) have unique advantages in improving medical and academic research. However, the research on the early embryo exposure of ultra-high SMFs is minimal, extensive exploration is indispensable in living organisms.
The present study was aimed to study the effects of ultra-high SMFs on the early embryonic division and development of Caenorhabditis elegans (C. elegans).
Early adult parents containing fertilized eggs in vivo were exposed to SMFs at intensities ranging from 4 T to 27 T. The number of mitotic cells in the reproductive glands of the P0 worms, early embryonic cell spindle localization, embryo hatching and the reproductive as well as developmental indicators of F1 and F2 nematodes were examined as endpoints.
Our results indicated that ultra-high SMFs has no obvious effect on the germ cell cycle, while 14 T and 27 T SMFs significantly increased the proportion of multi-polar spindle formation in early embryonic cells, and reduced the developmental rate and lifespan of C. elegans exposed at the embryonic stage. Spindle abnormalities of early embryonic cells, as well as the down-regulation of genes related to asymmetric embryonic division and the abnormal expression of the non-muscle myosin NMY-2 in the division grooves played a critical role in the slowing down of embryonic development induced by ultra-high SMFs.
This study provided novel information and a new sight for evaluating the biosafety assessment by exposure to ultra-high SMFs at the early embryonic stage in vivo.
超高静磁场（SMFs）在改善医学和学术研究方面具有独特优势。然而，关于超高SMFs对早期胚胎暴露的研究极少，在生物体中进行广泛探索必不可少。
本研究旨在研究超高SMFs对秀丽隐杆线虫（C. elegans）早期胚胎分裂和发育的影响。
将体内含有受精卵的成年早期亲本暴露于强度范围为4 T至27 T的SMFs中。以P0蠕虫生殖腺中的有丝分裂细胞数量、早期胚胎细胞纺锤体定位、胚胎孵化以及F1和F2线虫的生殖和发育指标作为终点进行检测。
我们的结果表明，超高SMFs对生殖细胞周期没有明显影响，而14 T和27 T的SMFs显著增加了早期胚胎细胞中多极纺锤体形成的比例，并降低了在胚胎期暴露的秀丽隐杆线虫的发育速率和寿命。早期胚胎细胞的纺锤体异常，以及与胚胎不对称分裂相关基因的下调和分裂沟中非肌肉肌球蛋白NMY-2的异常表达，在超高SMFs诱导的胚胎发育迟缓中起关键作用。
本研究为评估体内早期胚胎阶段暴露于超高SMFs的生物安全性评估提供了新的信息和视角。
