使用聚合酶链式反应（PCR）对感染犬只进行检测与鉴定
Detection and Identification of in Infected Dogs Using PCR.
作者信息
Öge H, Öge S, Özbakiş-Beceriklisoy G
机构信息
Ankara University, Faculty of Veterinary Medicine, Department of Parasitology, Ankara, Turkey.
出版信息
Helminthologia. 2019 Jun 1;56(2):118-123. doi: 10.2478/helm-2019-0008. eCollection 2019 Jun.
Faecal samples were collected from 224 dogs (47 villages) in Ankara. spp. eggs were diagnosed in faeces using centrifugal flotation and sedimentation methods. A total of 21 dogs (9.38 %) were positive for spp. eggs. In this study, we used the PCR technique that, in combination with DNA sequencing, allows the detection and identification of eggs in faeces of infected dogs. For this purpose, the ATPase subunit-6 gene (mtDNA) was selected as a target for the amplification . The primers were used to amplify 217 bp region. Amongst 21 coproscopically detected isolates from dogs, 5 (23.8 %) samples were PCR-positive for , and the remaining 16 samples were PCR-negative. Results indicate that PCR can detect DNA in faeces of infected dogs, but efficacy was low when compare to sedimentation/flotation. PCR is additional test for diagnosing of this infection. But, the difficulties of identification based on PCR in faecal examinations need to be investigated further.
从安卡拉的224只狗（来自47个村庄）采集粪便样本。使用离心浮选法和沉淀法在粪便中诊断出 属虫卵。共有21只狗（9.38%）的粪便中 属虫卵呈阳性。在本研究中，我们使用了聚合酶链反应（PCR）技术，该技术结合DNA测序，可检测和鉴定感染犬粪便中的 虫卵。为此，选择ATP酶亚基6基因（线粒体DNA）作为扩增靶点。引物用于扩增217 bp区域。在21份经粪便检查检测到的犬 分离株中，5份（23.8%）样本的PCR检测呈阳性，其余16份样本的PCR检测呈阴性。结果表明，PCR可检测感染犬粪便中的 属DNA，但与沉淀/浮选法相比，效率较低。PCR是诊断这种感染的辅助检测方法。但是，粪便检查中基于PCR进行鉴定的困难仍需进一步研究。
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