Ahmad Nazri Khuzaidatul Azidah, Fauzi Norsyahida Mohd, Buang Fhataheya, Mohd Saad Qodriyah Haji, Husain Khairana, Jantan Ibrahim, Jubri Zakiah
Department of Biochemistry, Faculty of Medicine, The National University of Malaysia Medical Centre (UKMMC), Kuala Lumpur, Malaysia.
Drug and Herbal Research Centre, Faculty of Pharmacy, The National University of Malaysia Campus Kuala Lumpur, Kuala Lumpur, Malaysia.
Evid Based Complement Alternat Med. 2019 Sep 23;2019:7246756. doi: 10.1155/2019/7246756. eCollection 2019.
(Lour.) Merr. (GP) has been reported in previous studies to possess antihyperlipidaemic, antioxidative, and cardioprotective properties. This study was aimed to determine the effect of standardised 80% ethanol extract of GP on lipid profiles and oxidative status of hypercholesterolemic rats. Postmenopausal (PM) Sprague-Dawley rats were ovariectomised and fed with 2% cholesterol diet fortified with five times heated palm oil to develop hyperlipidaemia status. Two doses of the extract (250 and 500 mg/kg) and atorvastatin (10 mg/kg) were administered once daily via oral gavage for 24 weeks. Systolic blood pressure (SBP) was increased during the first month in the postmenopausal group and decreased with GP supplementation. Lipid droplets accumulation was shown at the tunica media (TM) area of the aorta in the postmenopausal group and reduced with GP supplementation. Total cholesterol (TC), total triglycerides (TG), low-density lipoprotein (LDL), and malondialdehyde (MDA) levels increased ( < 0.05) at 3 and 6 months in the postmenopausal group and were reduced with GP supplementation. GP also increased high-density lipoprotein (HDL) level in the postmenopausal group. Superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) activities were reduced in the postmenopausal group compared to control in the sham group but increased ( < 0.05) with GP supplementation. The results showed that the higher dose of GP (500 mg/kg) gave better effect. GP has the ability to reduce oxidative stress and prevent membrane cell damage through antioxidant enzyme activity modification and lipid profile changes in postmenopausal rats related to atherosclerosis.
先前的研究报道，（ Lour.） Merr.（ GP）具有降血脂、抗氧化和心脏保护特性。本研究旨在确定标准化的 80%乙醇 GP 提取物对高胆固醇血症大鼠血脂谱和氧化状态的影响。对绝经后（ PM）的 Sprague-Dawley 大鼠进行卵巢切除，并喂食添加了五次加热棕榈油的 2%胆固醇饮食以诱导高脂血症状态。通过口服灌胃，每天一次给予两种剂量的提取物（ 250 和 500 mg/kg）以及阿托伐他汀（ 10 mg/kg），持续 24 周。绝经后组在第一个月收缩压（ SBP）升高，而补充 GP 后降低。绝经后组在主动脉中膜（ TM）区域出现脂滴积聚，补充 GP 后减少。绝经后组在 3 个月和 6 个月时总胆固醇（ TC）、总甘油三酯（ TG）、低密度脂蛋白（ LDL）和丙二醛（ MDA）水平升高（ P<0.05），补充 GP 后降低。 GP 还提高了绝经后组的高密度脂蛋白（ HDL）水平。与假手术组的对照组相比，绝经后组超氧化物歧化酶（ SOD）、过氧化氢酶（ CAT）和谷胱甘肽过氧化物酶（ GPx）活性降低，但补充 GP 后升高（ P<0.05）。结果表明，较高剂量的 GP（ 500 mg/kg）效果更好。 GP 具有通过改变抗氧化酶活性和改善与动脉粥样硬化相关的绝经后大鼠血脂谱来降低氧化应激和预防膜细胞损伤的能力。
