Early Detection of Colon Cancer Using Molecular and Microbial Markers (EC2M3)-EA 7375 Team, University Paris-Est Creteil (UPEC), Créteil, France.
Bioinformatic Core Facility, IMRB UMR 955 - INSERM, UPEC, Créteil Cedex, France.
PLoS One. 2021 Mar 9;16(3):e0247798. doi: 10.1371/journal.pone.0247798. eCollection 2021.
Extrahepatic cholangiocarcinoma (CCA) accounts for 3% of digestive cancers. The role of biliary microbiota as an environment-related modulator has been scarcely investigated in CCA, and the putative impact of associated diseases has not been yet assessed. We characterized the biliary microbiota in CCA patients in order to identify a specific CCA-related dysbiosis. The biliary effluents were collected through an endoscopic retrograde pancreatic cholangiography (ERCP) examination involving 28 CCA and 47 patients with gallstones, herein considered as controls. The biliary effluents were submitted to bacterial DNA extraction and 16S rRNA sequencing, using Illumina technology. Overall, 32% of CCA and 22% of controls displayed another associated disease, such as diabetes, pancreatitis, inflammatory bowel disease, or primary sclerosing cholangitis. Such associated diseases were considered in the comparisons that were made. Principal coordinate analysis (PCoA) detected a significant disparity of biliary microbiota composition between CCA patients and controls without an associated disease. Amongst the most abundant phyla, Proteobacteria did not significantly differ between CCA patients and controls, whereas Firmicutes levels were lower and Bacteroidetes higher in CCAs' biliary microbiota than in the controls' microbiota. The most abundant genera were Enterococcus, Streptococcus, Bacteroides, Klebsiella, and Pyramidobacter in CCA's biliary microbiota. Additionally, levels of Bacteroides, Geobacillus, Meiothermus, and Anoxybacillus genera were significantly higher in CCA patients' biliary microbiota, without an associated disease, in comparison with controls. A specific CCA-related dysbiosis was identified as compared to controls independently from associated diseases. This suggests that a microorganism community may be involved in CCA pathogenesis.
肝外胆管癌（CCA）占消化系统癌症的 3%。胆道微生物群作为一种与环境相关的调节剂，其在 CCA 中的作用尚未得到充分研究，相关疾病的潜在影响也尚未评估。我们对 CCA 患者的胆道微生物群进行了特征描述，以确定一种特定的与 CCA 相关的菌群失调。通过内镜逆行胰胆管造影（ERCP）检查收集胆道流出物，该检查涉及 28 例 CCA 患者和 47 例胆石症患者，后者被认为是对照组。对胆道流出物进行细菌 DNA 提取和 16S rRNA 测序，使用 Illumina 技术。总体而言，32%的 CCA 患者和 22%的对照组患有另一种相关疾病，如糖尿病、胰腺炎、炎症性肠病或原发性硬化性胆管炎。在进行比较时考虑了这些相关疾病。主坐标分析（PCoA）检测到 CCA 患者和无相关疾病的对照组之间胆道微生物群组成存在显著差异。在最丰富的门中，变形菌门在 CCA 患者和对照组之间没有显著差异，而厚壁菌门水平在 CCA 患者的胆道微生物群中较低，拟杆菌门水平较高。CCA 胆道微生物群中最丰富的属为肠球菌属、链球菌属、拟杆菌属、克雷伯菌属和Pyramidobacter 属。此外，在无相关疾病的情况下，CCA 患者胆道微生物群中Bacteroides、Geobacillus、Meiothermus 和 Anoxybacillus 属的水平显著高于对照组。与对照组相比，CCA 患者胆道微生物群中存在一种特定的与 CCA 相关的菌群失调，独立于相关疾病。这表明微生物群落可能参与了 CCA 的发病机制。
