伊朗出现对特比萘芬耐药的情况，其角鲨烯环氧酶（）基因存在突变。
Emergence of Terbinafine Resistant in Iran, Harboring Mutations in the Squalene Epoxidase () Gene.
作者信息
Taghipour Simin, Shamsizadeh Forough, Pchelin Ivan M, Rezaei-Matehhkolaei Ali, Zarei Mahmoudabadi Ali, Valadan Reza, Ansari Saham, Katiraee Farzad, Pakshir Keyvan, Zomorodian Kamiar, Abastabar Mahdi
机构信息
Department of Medical Parasitology and Mycology, Faculty of Medicine, Shahrekord University of Medical Sciences, Shahrekord, Iran.
Student Research Committee, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
出版信息
Infect Drug Resist. 2020 Mar 13;13:845-850. doi: 10.2147/IDR.S246025. eCollection 2020.
INTRODUCTION
and are important causative agents of superficial mycoses, demonstrating emergent antifungal drug resistance. We studied the antifungal susceptibility profiles in Iranian isolates of these two species.
METHODS
A total of 96 and 45 isolates were subjected to molecular typing by ribosomal ITS region. Antifungal susceptibility profiles for terbinafine, griseofulvin, clotrimazole, efinaconazole, luliconazole, amorolfine and ciclopirox were obtained by CLSI broth microdilution method. The squalene epoxidase () gene was subjected to sequencing for mutations, if any, in isolates exhibiting elevated MICs for terbinafine.
RESULTS
Luliconazole and efinaconazole showed the lowest MIC values against and isolates. There were five isolates with terbinafine MICs ≥32 µg/mL in our sample. They belonged to type VIII and harbored two alternative gene sequence variants, leading to Phe397Leu and Ala448Thr or Leu393Ser and Ala448Thr substitutions in the enzyme. All terbinafine resistant strains could be inhibited by luliconazole and efinaconazole.
CONCLUSION
This study documented a step in the global spread of resistance mechanisms in . However, treatment alternatives for resistant isolates were available.
引言
[两种真菌名称未给出]是浅表真菌病的重要病原体，表现出新兴的抗真菌药物耐药性。我们研究了这两个菌种的伊朗分离株的抗真菌药敏谱。
方法
共96株[第一种真菌名称未给出]和45株[第二种真菌名称未给出]分离株通过核糖体ITS区域进行分子分型。采用CLSI肉汤微量稀释法获得特比萘芬、灰黄霉素、克霉唑、艾氟康唑、卢立康唑、阿莫罗芬和环吡酮的抗真菌药敏谱。对特比萘芬MIC值升高的分离株的角鲨烯环氧酶（[基因名称未给出]）基因进行测序以检测是否存在突变。
结果
卢立康唑和艾氟康唑对[第一种真菌名称未给出]和[第二种真菌名称未给出]分离株显示出最低的MIC值。我们的样本中有5株特比萘芬MIC值≥32 µg/mL的分离株。它们属于[第一种真菌名称未给出]VIII型，具有两个替代的[基因名称未给出]基因序列变体，导致该酶中出现Phe397Leu和Ala448Thr或Leu393Ser和Ala448Thr替代。所有特比萘芬耐药菌株均可被卢立康唑和艾氟康唑抑制。
结论
本研究记录了[第一种真菌名称未给出]耐药机制在全球传播的一个情况。然而，耐药分离株有替代治疗方案。
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