Wei Xinlei, You Chun
Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China.
Sheng Wu Gong Cheng Xue Bao. 2019 Oct 25;35(10):1870-1888. doi: 10.13345/j.cjb.190213.
In vitro multi-enzyme molecular machines that follow the designed multi-enzyme pathways, require the rational optimization and adaptation of several purified or partially purified enzyme components, in order to convert certain substrates into target compounds in vitro in an efficient manner. This type of molecular machine is component-based and modularized, so that its design, assembly, and regulation processes are highly flexible. Recently, the advantages of in vitro multi-enzyme molecular machines on the precise control of reaction process and the enhancement of product yield have suggested their great application potential in biomanufacturing. Studies on in vitro multi-enzyme molecular machines have become an important branch of synthetic biology, and are gaining increasing attentions. This article systematically reviews the enzyme component-/module-based construction strategy of in vitro multi-enzyme molecular machines, as well as the research progress on the improvement of compatibility among enzyme components/modules. The current challenges and future prospects of in vitro multi-enzyme molecular machines are also discussed.
遵循设计的多酶途径的体外多酶分子机器，需要对几种纯化的或部分纯化的酶组分进行合理优化和适配，以便在体外高效地将某些底物转化为目标化合物。这类分子机器基于组件且模块化，因此其设计、组装和调控过程具有高度灵活性。近来，体外多酶分子机器在精确控制反应过程和提高产物产量方面的优势，显示出它们在生物制造中的巨大应用潜力。对体外多酶分子机器的研究已成为合成生物学的一个重要分支，并日益受到关注。本文系统综述了基于酶组分/模块构建体外多酶分子机器的策略，以及提高酶组分/模块之间兼容性的研究进展。还讨论了体外多酶分子机器当前面临的挑战和未来前景。
