Zhejiang Provincial Key Laboratory of Horticultural Plant Integrative Biology, Zhejiang University, Hangzhou 310058, China.
Zhejiang Provincial Key Laboratory of Horticultural Plant Integrative Biology, Zhejiang University, Hangzhou 310058, China.
Food Chem. 2020 Mar 30;309:125705. doi: 10.1016/j.foodchem.2019.125705. Epub 2019 Oct 21.
Red-peeled huyou has a distinct red peel color due mainly to the presence of red apocarotenoid β-citraurin as well as the increase in amount of total carotenoids. The expression level of carotenoid cleavage dioxygenase 4b1 (CCD4b1) accounted for 99.0% of total transcript abundance of CCD4s in red-peeled huyou peel and was nearly 100 times higher than that in ordinary huyou. β-Citraurin accumulation and peel coloration was mostly favored at 15 °C but strongly inhibited at moderately high temperatures 20 °C and 25 °C. Exogenous ethylene application for 3 d had no obvious effect on β-citraurin accumulation in red-peeled huyou but holding fruit at moderately higher temperatures (20 °C and 25 °C) for 3 d had a significant adverse effect on β-citraurin accumulation. The expression of phytoene synthase 1 (PSY1) and CCD4b1 was higher at 10 °C and 15 °C and significantly lower at 20 °C and 25 °C. The mechanisms governing the accumulation of β-citraurin are discussed.
红皮厚皮甜瓜的果皮呈现明显的红色，主要归因于存在红色类胡萝卜素β-隐黄质以及总类胡萝卜素含量的增加。类胡萝卜素裂解双加氧酶 4b1（CCD4b1）的表达水平占 CCD4s 总转录丰度的 99.0%，在红皮厚皮甜瓜果皮中几乎是普通厚皮甜瓜的 100 倍。β-隐黄质的积累和果皮着色主要在 15°C 时受到促进，但在中等高温 20°C 和 25°C 时受到强烈抑制。外源乙烯处理 3 天对红皮厚皮甜瓜中β-隐黄质的积累没有明显影响，但将果实置于中等偏高的温度（20°C 和 25°C）下 3 天对β-隐黄质的积累有显著的不利影响。在 10°C 和 15°C 时，八氢番茄红素合酶 1（PSY1）和 CCD4b1 的表达水平较高，在 20°C 和 25°C 时显著降低。讨论了β-隐黄质积累的机制。
