基于光致增强拉曼光谱的多功能生物分子检测平台。
A versatile biomolecular detection platform based on photo-induced enhanced Raman spectroscopy.
机构信息
Shanghai Key Laboratory of Green Chemistry and Chemical Processes, School of Chemistry and Molecular Engineering, East China Normal University, 500 Dongchuan Road, Shanghai, 200241, PR China.
The RNA Institute, University at Albany, State University of New York, Albany, NY 12222, USA.
出版信息
Biosens Bioelectron. 2020 Jan 1;147:111742. doi: 10.1016/j.bios.2019.111742. Epub 2019 Oct 2.
Surface-enhanced Raman spectroscopy (SERS) as one of the effective tools for sensitive and selective detection of biomolecules has attracted tremendous attention. Here, we construct a versatile biomolecular detection platform based on photo-induced enhanced Raman spectroscopy (PIERS) effect for ultrasensitive detection of multiple analytes. In our PIERS sensor, we exploit the molecular recognition capacity of aptamers and the high affinity of aptamers with analyte to trigger TiO@AgNP substrates binding with Raman tag-labeled gold nanoparticles probes via analyte, thus forming sandwich complexes. Additionally, combining plasmonic nanoparticles with photo-activated substrates allows PIERS sensor to achieve increased sensitivity beyond the normal SERS effect upon ultraviolet irradiation. Accordingly, the PIERS can be implemented for analysis of multiple analytes by designing different analyte aptamers, and we further demonstrate that the constructed PIERS sensor can serve as a versatile detection platform for sensitively analyzing various biomolecules including small molecules (adenosine triphosphate (ATP), limit of detection (LOD) of 0.1 nM), a biomarker (thrombin, LOD of 50 pM), and a drug (cocaine, LOD of 5 nM). Therefore, this versatile biomolecular detection platform based on PIERS effect for ultrasensitive detection of multiple analytes holds great promise to be a practical tool.
表面增强拉曼光谱（SERS）作为一种用于灵敏和选择性检测生物分子的有效工具，引起了极大的关注。在这里，我们构建了一种基于光诱导增强拉曼光谱（PIERS）效应的多功能生物分子检测平台，用于超灵敏检测多种分析物。在我们的 PIERS 传感器中，我们利用适体的分子识别能力和适体与分析物的高亲和力，通过分析物触发 TiO@AgNP 基底与拉曼标记的金纳米粒子探针结合，从而形成三明治复合物。此外，将等离子体纳米粒子与光激活基底结合使用，可以使 PIERS 传感器在紫外光照射下实现超出正常 SERS 效应的灵敏度增强。因此，通过设计不同的分析物适体，我们可以将 PIERS 用于分析多种分析物，并且我们进一步证明，所构建的 PIERS 传感器可以作为一种通用的检测平台，用于灵敏地分析各种生物分子，包括小分子（三磷酸腺苷（ATP），检测限（LOD）为 0.1 nM）、生物标志物（凝血酶，LOD 为 50 pM）和药物（可卡因，LOD 为 5 nM）。因此，基于 PIERS 效应的多功能生物分子超灵敏检测平台有望成为一种实用工具。
Zotero 插件