Raucci Ada, Sorrentino Giuseppina, Singh Sima, Borbone Nicola, Oliviero Giorgia, Piccialli Gennaro, Terracciano Monica, Cinti Stefano
Department of Pharmacy, University of Naples "Federico II, " Via Domenico Montesano 49, 80131, Naples, Italy.
Department of Molecular Medicine and Medical Biotechnologies, University of Naples Federico II, via Sergio Pansini 5, 80131, Naples, Italy.
Anal Bioanal Chem. 2025 Apr;417(9):1863-1872. doi: 10.1007/s00216-025-05764-9. Epub 2025 Feb 1.
Thrombin overexpression in serum serves as a critical biomarker and is implicated in several diseases associated with significant morbidity and mortality. Existing techniques for thrombin detection are time-consuming and require sophisticated equipment and extensive sample preparation procedures, which further delay the detection and increase the cost of the procedure. Early and accessible diagnosis at the point of care, especially in limited-resource countries, represents the first step of clinical interventions. To overcome these limitations, we have proposed an innovative, sustainable paper-based electrochemical detection platform for thrombin. In this work, a sustainable paper-based aptasensor was rationally designed, characterized, evaluated against conventional gold standard plastic-based substrates, and applied to human serum, yielding a detection limit of ~ 60 pM. The present method provides an efficient and user-friendly way for the detection of thrombin and potentially leading to better management and treatment outcomes for patients.
血清中凝血酶的过表达是一种关键的生物标志物，与几种具有显著发病率和死亡率的疾病有关。现有的凝血酶检测技术耗时且需要精密设备和大量样品制备程序，这进一步延迟了检测并增加了检测成本。在护理点进行早期且便捷的诊断，尤其是在资源有限的国家，是临床干预的第一步。为克服这些限制，我们提出了一种用于凝血酶检测的创新型、可持续的纸质电化学检测平台。在这项工作中，合理设计了一种可持续的纸质适配体传感器，对其进行了表征，与传统的基于塑料的金标准底物进行了评估，并应用于人体血清，检测限约为60 pM。本方法为凝血酶检测提供了一种高效且用户友好的方式，并可能为患者带来更好的管理和治疗效果。
