Shahbazi Gholamreza, Hoghooghi Rad Nasser, Madani Rassoul, Matin Somaieh, Mortazavi Pedjman, Jangjou Amir Hossein
Department of Parasitology and Mycology, Faculty of Specialized Veterinary Science, Islamic Azad University, Science and Research Branch, Tehran, Iran.
Department of Clinical Pathology, Faculty of Specialized Veterinary Science, Islamic Azad University, Science and Research Branch, Tehran, Iran.
Iran J Parasitol. 2019 Jul-Sep;14(3):430-435.
One of the severe complications of toxoplasmosis is the induction of abortion in sheep, goats, and human beings. The rate of abortions related to toxoplasmosis in sheep varies between 10% and 23% in the United States and European countries. In Iran, infection were diagnosed in aborted ovine fetuses between 13.5% and 69% in the brain samples based on PCR. The purpose of this study was to investigate the prevalence of abortions related to among the aborted fetuses of sheep in Ardabil area, North-West of Iran.
The brains of 75 aborted sheep fetuses in Ardebil area were investigated with nested PCR method using fragments of the GRA6 gene during lambing seasons in 2014-2015. Meanwhile, thoracic and abdominal fluids of aborted fetuses and 200 serum samples collected from the herds affected by abortion were examined for the existence of anti- IgG.
For 48 out of the 75 fetal brain samples (64%), the results of nested PCR test were found to be positive. Furthermore, antibodies against was observed in 136 (68%) collected samples of sheep and 21 samples (28%) of fetal fluids.
There is the significant role of in the abortions of sheep in Ardabil area. Meanwhile, this condition can also be dangerous for human beings because of their consumption of sheep meat.
弓形虫病的严重并发症之一是导致绵羊、山羊和人类流产。在美国和欧洲国家，与弓形虫病相关的绵羊流产率在10%至23%之间。在伊朗，基于聚合酶链反应（PCR），在流产绵羊胎儿的脑样本中，弓形虫感染率在13.5%至69%之间。本研究的目的是调查伊朗西北部阿尔达比勒地区绵羊流产胎儿中与弓形虫病相关的流产患病率。
在2014 - 2015年产羔季节，采用巢式PCR方法对阿尔达比勒地区75只流产绵羊胎儿的大脑进行检测，所用引物针对GRA6基因片段。同时，检测流产胎儿的胸腹水以及从受流产影响的羊群中采集的200份血清样本中抗弓形虫IgG的存在情况。
75份胎儿脑样本中有48份（64%）巢式PCR检测结果呈阳性。此外，在采集的136份（68%）绵羊样本和21份（28%）胎儿液体样本中检测到抗弓形虫抗体。
弓形虫在阿尔达比勒地区绵羊流产中起重要作用。同时，由于人类食用羊肉，这种情况对人类也可能构成危险。
