Key Laboratory of Hunan Province for Water Environment and Agriculture Product Safety, College of Chemistry and Chemical Engineering, Central South University, Changsha, 410083, China.
Mikrochim Acta. 2019 Oct 31;186(11):735. doi: 10.1007/s00604-019-3834-6.
Prussian Blue nanoparticles were deposited on g-CN nanosheets. The resulting nanocomposite possesses peroxidase-like (POx) activity and can catalyze the oxidation of 3,3',5,5'-tetramethylbenzidine at room temperature in the presence of HO. This leads to formation of a blue product with an absorption maximum at 650 nm. The formation of the Prussian Blue nanoparticles on the g-CN nanosheets, and the POx-like activity of the composite were characterized in detail. The POx mimic was used for determination of L-lactic acid via detection of HO that is produced by the enzyme lactate oxidase (LOx). The assay has a linear range that extends from 5 to 100 μM, and the detection limit is 2.2 μM. The method was successfully applied to the determination of L-lactic acid in spiked human serum. Graphical abstract Ultra-small Prussian Blue (PB) nanoparticles were used to modify g-CN nanosheets, and their peroxidase-like activity was explored for detection of L-lactic acid. LOx represent L-lactate oxidase, and TMB represents 3,3',5,5'-tetramethylbenzidine.
普鲁士蓝纳米粒子沉积在 g-CN 纳米片上。所得的纳米复合材料具有过氧化物酶样（POx）活性，并且可以在室温下在 HO 的存在下催化 3,3',5,5'-四甲基联苯胺的氧化。这导致在 650nm 处具有最大吸收的蓝色产物的形成。详细表征了普鲁士蓝纳米粒子在 g-CN 纳米片上的形成以及该复合材料的 POx 样活性。通过检测由酶乳酸氧化酶（LOx）产生的 HO 来使用过氧化物酶模拟物测定 L-乳酸。该测定法的线性范围从 5 到 100μM 延伸，检测限为 2.2μM。该方法成功地应用于测定加标人血清中的 L-乳酸。 示意图 超小的普鲁士蓝（PB）纳米粒子用于修饰 g-CN 纳米片，并探索了其过氧化物酶样活性以用于检测 L-乳酸。LOx 代表 L-乳酸氧化酶，而 TMB 代表 3,3',5,5'-四甲基联苯胺。
