Krug Benedikt, Koukourakis Nektarios, Czarske Juergen W
Opt Express. 2019 Sep 16;27(19):26910-26923. doi: 10.1364/OE.27.026910.
The mechanical properties of tissues and cells are increasingly recognized as an important feature for the understanding of pathological processes and as a diagnostic tool in biomedicine. Impulsive stimulated Brillouin scattering (ISBS) is promising to overcome shortcomings of other measurement methods such as invasiveness, low spatial resolution and long acquisition time. In this paper, we present for the first time ISBS measurements of hydrogels, which are model materials for biological samples. We demonstrate ISBS measurements discriminating hydrogels of different stiffness. ISBS measurements with lateral resolution close to cellular level are presented. These results underline that ISBS microscopy has a high potential for biomedical applications.
组织和细胞的力学特性越来越被认为是理解病理过程的一个重要特征，也是生物医学中的一种诊断工具。脉冲激发布里渊散射（ISBS）有望克服其他测量方法的缺点，如侵入性、低空间分辨率和长采集时间。在本文中，我们首次展示了对水凝胶的ISBS测量，水凝胶是生物样本的模型材料。我们展示了能够区分不同刚度水凝胶的ISBS测量。还展示了横向分辨率接近细胞水平的ISBS测量。这些结果表明，ISBS显微镜在生物医学应用方面具有很大潜力。
