Le Taoran, Li Jiarui, Wei Haoyun, Li Yan
State Key Laboratory of Precision Measurement Technology and Instruments, Department of Precision Instrument, Tsinghua University, Beijing 100084, China.
Photoacoustics. 2025 Feb 10;42:100696. doi: 10.1016/j.pacs.2025.100696. eCollection 2025 Apr.
Brillouin microscopy has been widely used in the mechanical imaging of cells and tissues, and the signal-to-noise (SNR) ratio limits the spectral integration time. Impulsive stimulated Brillouin scattering (ISBS) microscopy is a new elastic imaging technique. As a variant of stimulated Brillouin scattering (SBS), ISBS can overcome the weak signal of spontaneous Brillouin scattering. A simple model can estimate SBS gain. However, the theoretical ISBS gain has not been compared with SBS gain. This paper gives the theoretical ISBS gain estimation, and experiments are designed to verify estimation reliability. The heterodyne ISBS gain coefficient can be much higher than SBS gain coefficient. The relationship between ISBS gain coefficient and spatial resolution is then discussed. We anticipate that the ISBS setup optimization can improve spatial resolution and gain, potentially enabling fast and high spatial resolution imaging of biological cells.
布里渊显微镜已广泛应用于细胞和组织的力学成像，而信噪比限制了光谱积分时间。脉冲受激布里渊散射（ISBS）显微镜是一种新的弹性成像技术。作为受激布里渊散射（SBS）的一种变体，ISBS可以克服自发布里渊散射的弱信号。一个简单的模型可以估计SBS增益。然而，理论上的ISBS增益尚未与SBS增益进行比较。本文给出了理论ISBS增益估计，并设计实验验证估计的可靠性。外差ISBS增益系数可比SBS增益系数高得多。然后讨论了ISBS增益系数与空间分辨率之间的关系。我们预计，ISBS装置的优化可以提高空间分辨率和增益，有可能实现生物细胞的快速高空间分辨率成像。
