Opt Express. 2022 Feb 14;30(4):4748-4758. doi: 10.1364/OE.449980.
The impulsive stimulated Brillouin microscopy promises fast, non-contact measurements of the elastic properties of biological samples. The used pump-probe approach employs an ultra-short pulse laser and a cw laser to generate Brillouin signals. Modeling of the microscopy technique has already been carried out partially, but not for biomedical applications. The nonlinear relationship between pulse energy and Brillouin signal amplitude is proven with both simulations and experiments. Tayloring of the excitation parameters on the biologically relevant polyacrylamide hydrogels outline sub-ms temporal resolutions at a relative precision of <1%. Brillouin microscopy using the impulsive stimulated scattering therefore exhibits high potential for the measurements of viscoelastic properties of cells and tissues.
脉冲刺激布里渊显微镜有望实现对生物样本弹性特性的快速、非接触测量。所采用的泵浦-探测方法使用超短脉冲激光和连续波激光来产生布里渊信号。该显微镜技术的建模已经部分完成，但不适用于生物医学应用。通过模拟和实验证明了脉冲能量与布里渊信号幅度之间的非线性关系。在生物相关的聚丙烯酰胺水凝胶上对激发参数进行调整，可在相对精度<1%的情况下实现亚毫秒级的时间分辨率。因此，使用脉冲刺激散射的布里渊显微镜在测量细胞和组织的粘弹性特性方面具有很大的潜力。
