Department of Periodontology, Stomatological Hospital , Jilin University , Changchun 130021 , P. R. China.
State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry , Chinese Academy of Sciences , Changchun 130022 , P. R. China.
Langmuir. 2019 Nov 26;35(47):15287-15294. doi: 10.1021/acs.langmuir.9b02824. Epub 2019 Nov 12.
Effective bone tissue reconstitution improves the treatment success rate of dental implantation and preserves natural teeth during periodontal tissue repair. Hydroxyapatite (HAp) has received much attention in bone remodeling field because its mineralized structure is similar to that of the natural bone tissue. For this reason, it has been used as a carrier for growth factors. Although HAp possesses outstanding biomedical properties, its capacity of loading and releasing bone growth factors and promoting osteogenesis is not well understood. In this study, Ln (Ln = Yb, Er, Gd)-doped HAp (HAp:Ln) nanorods were synthesized by one-step hydrothermal method. To improve its biocompatibility and surface properties, bone morphogenetic protein-2 (BMP-2) was loaded onto the surface of HAp:Ln nanorods. The results showed that BMP-2 incorporation promoted bone formation and enhanced the expression of early bone-related gene and protein (RunX2, SP7, OPN). In addition, Yb- and Er-doped HAp nanorods were examined by upconversion luminescence with 980 nm near-infrared laser irradiation to monitor the delivery position of BMP-2 protein. Furthermore, due to the positive magnetism correlated with the concentration of Gd, HAp:Ln with enhanced contrast brightening can be deemed as MIR contrast agents. These findings indicate that HAp doped with rare-earth ions and loaded with BMP-2 has the potential to promote bone tissue repair and execute dual-mode imaging.
有效骨组织重建可提高牙种植体治疗成功率，并在牙周组织修复过程中保留天然牙齿。羟基磷灰石（HAp）因其矿化结构与天然骨组织相似，在骨重塑领域受到广泛关注。因此，它被用作生长因子的载体。尽管 HAp 具有出色的生物医学特性，但人们对其加载和释放骨生长因子以及促进成骨的能力了解甚少。在这项研究中，通过一步水热法合成了 Ln（Ln=Yb、Er、Gd）掺杂的 HAp（HAp：Ln）纳米棒。为了提高其生物相容性和表面性能，将骨形态发生蛋白-2（BMP-2）负载到 HAp：Ln 纳米棒的表面上。结果表明，BMP-2 的掺入促进了骨形成，并增强了早期骨相关基因和蛋白（RunX2、SP7、OPN）的表达。此外，通过 980nm 近红外激光照射的上转换发光对 Yb 和 Er 掺杂的 HAp 纳米棒进行了检测，以监测 BMP-2 蛋白的递送位置。此外，由于 Gd 浓度与正磁相关性，HAp：Ln 具有增强的对比增亮效果，可以被视为 MIR 造影剂。这些发现表明，掺杂稀土离子并负载 BMP-2 的 HAp 有望促进骨组织修复并执行双模成像。
