State Key Laboratory of Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, PR China.
Department of Epidemiology, Shanxi Medical University School of Public Health, Taiyuan, Shanxi 030001, PR China.
Int J Syst Evol Microbiol. 2020 Feb;70(2):744-750. doi: 10.1099/ijsem.0.003807.
Two strains, designated 2251 and 3058, that were aerobic, Gram-stain-negative, non-motile, coccoid or short rod-shaped bacilli, have recently been isolated from Tibetan antelopes on the Qinghai-Tibet Plateau. The results of phylogenetic analyses of 16S rRNA gene sequences indicated that strains 2251 and 3058 represent a new species within the genus and are most similar to '' CUG00006 (98.9 and 99.3 %), I-41R45 (98.3 and 98.7 %) and THG-T2.31 (97.6 and 97.8 %). Results of genomic sequence-based phylogenomic analysis agreed with those from 16S rRNA gene sequence analysis. Optimal growth was achieved at pH 7.0-7.5 and 28 °C with marine medium. Cells contained C 7 as the major cellular fatty acid and ubiquinone-10 as the predominant menaquinone. The polar lipids comprised phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, phospholipid, glycolipid and an unidentified lipid. The cell-wall peptidoglycan amino acids were meso-2,6-diaminopimelic acid, alanine and glutamic acid; the major cell-wall sugar was galactose. The G+C content of strain 2251 was 66.5 mol%. Both strains (2251 and 3058) had DNA-DNA relatedness values less than 50 % with all available genomes of the genus in the ncbi database. Differential genotypic inferences, together with phenotypic and biochemical characteristics, demonstrated that strains 2251 and 3058 should be classified as a novel species of the genus , for which the name sp. nov. is suggested. The type strain is 2251 (=CGMCC 1.16490=DSM 106269).
两株菌，分别命名为 2251 和 3058，它们是从青藏高原上的藏羚羊中分离出来的，属于需氧、革兰氏阴性、非运动、球形或短杆状杆菌。16S rRNA 基因序列的系统发育分析结果表明，菌株 2251 和 3058 代表了属内的一个新种，与“CUG00006（98.9%和 99.3%）、I-41R45（98.3%和 98.7%）和 THG-T2.31（97.6%和 97.8%）最为相似。基因组序列基于系统发育分析的结果与 16S rRNA 基因序列分析的结果一致。最佳生长条件为 pH7.0-7.5 和 28°C，使用海洋培养基。细胞含有 C7 作为主要细胞脂肪酸和泛醌-10 作为主要menaquinone。极性脂包括磷脂酰乙醇胺、双磷脂酰甘油、磷脂酰甘油、磷脂、糖脂和一种未鉴定的脂。细胞壁肽聚糖的氨基酸为 meso-2,6-二氨基庚二酸、丙氨酸和谷氨酸；主要细胞壁糖是半乳糖。菌株 2251 的 G+C 含量为 66.5mol%。两株菌（2251 和 3058）与 ncbi 数据库中可获得的属内所有基因组的 DNA-DNA 同源性值均小于 50%。差异基因型推断，结合表型和生化特征，表明菌株 2251 和 3058 应归类为属的一个新种，建议命名为 。模式菌株为 2251（=CGMCC 1.16490=DSM 106269）。
