Li Junqin, Lei Wenjing, Yang Jing, Lu Shan, Jin Dong, Lai Xin-He, Zhang Sihui, Cheng Yanpeng, Mi Fei, Huang Yuyuan, Pu Ji, Dong Kui, Tian Zhi, Wu Xiaomin, Huang Ying, Wang Suping, Xu Jianguo
State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, PR China.
Department of Epidemiology, Shanxi Medical University School of Public Health, Taiyuan, Shanxi 030001, PR China.
Int J Syst Evol Microbiol. 2020 Aug;70(8):4683-4690. doi: 10.1099/ijsem.0.004331.
Four novel strains (592, S592, MF47 and SMF47) were isolated from Tibetan antelopes () and plateau pikas (), respectively. The cells were aerobic, non-motile, Gram-stain- and catalase-positive, rod-shaped bacteria. The 16S rRNA gene sequences of the four strains showed highest similarities to DSM 10552 (98.1, 98.6, 98.7 and 98.7 %, respectively), and the phylogenetic analyses based on 16S rRNA gene and genomic sequences indicated that strains 592 and MF47 represent two novel species. The four isolates produced acid from l-rhamnose, d-xylose and cellobiose, but were unable to reduce nitrate. The DNA G+C contents of strains 592 and MF47 were 70.3 and 69.8 mol%, respectively. The digital DNA-DNA hybridization value between strains 592 and MF47 was 32.6 %, lower than the threshold of 70 %, indicating they belong to different species. The four strains' genomes displayed less than 24.6 % DNA-DNA relatedness with all available genomes of the genus in the NCBI database, including NBRC 14897 and JCM 14732. The major fatty acids of the four strains were C ω9 and C 10-methyl, and the main polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol. The predominant respiratory quinones were MK-9(H) and MK-8(H). The cell-wall peptidoglycan contained ll-diaminopimelic acid. Based on these genotypic, phenotypic and biochemical analyses, it is proposed that the four unidentified bacteria be classified as two novel species, sp. nov. and sp. nov. The type strains are 592 (=CGMCC1.16526=DSM 106289) and MF47 (=CGMCC 1.17444=JCM 33790), respectively.
分别从藏羚羊和高原鼠兔中分离出四株新菌株(592、S592、MF47和SMF47)。这些细胞为需氧菌,无运动性,革兰氏染色和过氧化氢酶呈阳性,为杆状细菌。这四株菌株的16S rRNA基因序列与DSM 10552的相似性最高(分别为98.1%、98.6%、98.7%和98.7%),基于16S rRNA基因和基因组序列的系统发育分析表明,菌株592和MF47代表两个新物种。这四株分离菌可从L-鼠李糖、D-木糖和纤维二糖产酸,但不能还原硝酸盐。菌株592和MF47的DNA G+C含量分别为70.3 mol%和69.8 mol%。菌株592和MF47之间的数字DNA-DNA杂交值为32.6%,低于70%的阈值,表明它们属于不同的物种。这四株菌株的基因组与NCBI数据库中该属所有可用基因组(包括NBRC 14897和JCM 14732)的DNA-DNA相关性低于24.6%。这四株菌株的主要脂肪酸为C ω9和C 10-甲基,主要极性脂为双磷脂酰甘油、磷脂酰甘油和磷脂酰肌醇。主要呼吸醌为MK-9(H)和MK-8(H)。细胞壁肽聚糖含有ll-二氨基庚二酸。基于这些基因型、表型和生化分析,建议将这四株未鉴定细菌分类为两个新物种,即新种和新种。模式菌株分别为592(=CGMCC1.16526=DSM 106289)和MF47(=CGMCC 1.17444=JCM 33790)。