Department of Epidemiology, Shanxi Medical University School of Public Health, Taiyuan, Shanxi 030001, PR China.
Research Units of Discovery of Unknown Bacteria and Function, Chinese Academy of Medical Sciences, Beijing, PR China.
Int J Syst Evol Microbiol. 2020 Apr;70(4):2204-2210. doi: 10.1099/ijsem.0.003939. Epub 2020 Feb 7.
Two Gram-stain-positive, catalase-positive and oxidase-negative, aerobic, non-motile, cellobiose-utilizing, short-rod-shaped strains (Z28 and Z29) were isolated from faeces of Tibetan antelope () collected on the Qinghai-Tibet Plateau. Strain Z28 shared 98.1, 98.0, 97.8 and 97.4 % 16S rRNA gene similarity, 24.1, 22.8, 23.2 and 26.3 % digital DNA-DNA hybridization relatedness and 80.8, 80.0, 80.7 and 80.9 % average nucleotide identity values with DSM 24482, DSM 20109, DSM 14785 and JCM 14899, respectively. Results from further phylogenetic analyses based on the 16S rRNA gene and 148 core genes indicated that strains Z28 and Z29 were closest to DSM 24482 and DSM 20109, but clearly separated from the currently recognized species of the genus . The genomic DNA G+C content of strain Z28 was 75.3 mol%. The major cellular fatty acids were -C, -C A, C and -C. Ribose and mannose were detected as the whole-cell sugars. The major respiratory quinone was MK-9(H) and ornithine was the diamino acid of the cell wall. The polar lipids present in strain Z28 were phosphatidylethanolamine, five phospholipids, two aminophospholipids, aminolipid and three unidentified lipids. Comparison of phenotypic and phylogenetic features between the two strains and the related organisms revealed that Z28 and Z29 represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is Z28 (=CGMCC 1.16477=DSM 106200).
从青藏高原采集的藏羚羊粪便中分离到两株革兰氏阳性、过氧化氢酶阳性、氧化酶阴性、需氧、非运动、利用纤维二糖的短杆状菌株(Z28 和 Z29)。菌株 Z28 与 DSM 24482、DSM 20109、DSM 14785 和 JCM 14899 的 16S rRNA 基因相似度分别为 98.1%、98.0%、97.8%和 97.4%,数字 DNA-DNA 杂交相关性分别为 24.1%、22.8%、23.2%和 26.3%,平均核苷酸同一性值分别为 80.8%、80.0%、80.7%和 80.9%。基于 16S rRNA 基因和 148 个核心基因的进一步系统发育分析结果表明,菌株 Z28 和 Z29 与 DSM 24482 和 DSM 20109 最为接近,但与目前公认的属种明显分离。菌株 Z28 的基因组 DNA G+C 含量为 75.3mol%。主要细胞脂肪酸为 -C、-C A、C 和 -C。检测到核糖和甘露糖作为整个细胞的糖。主要呼吸醌为 MK-9(H),细胞壁中的二氨基酸为鸟氨酸。菌株 Z28 中存在的极性脂质有磷脂酰乙醇胺、五种磷脂、两种氨基磷脂、氨基脂和三种未鉴定的脂质。对两株菌和相关生物的表型和系统发育特征进行比较,发现 Z28 和 Z29 代表了一个新的种,属,提议使用 sp. nov. 作为新种的名称。模式菌株为 Z28(=CGMCC 1.16477=DSM 106200)。