Zhu Wentao, Yang Jing, Lu Shan, Lai Xin-He, Jin Dong, Pu Ji, Wang Xiaoxia, Huang Yuyuan, Zhang Sihui, Huang Ying, Tao Yuanmeihui, Ren Zhihong, Wu Xiaomin, Zhang Xiaoyan, Xu Jianqing, Xu Jianguo
Shanghai Institute for Emerging and Re-emerging Infectious Diseases, Shanghai Public Health Clinical Center, Shanghai 201508, PR China.
Research Units of Discovery of Unknown Bacteria and Function, Chinese Academy of Medical Sciences, Beijing 100730, PR China.
Int J Syst Evol Microbiol. 2019 Sep;69(9):2942-2947. doi: 10.1099/ijsem.0.003586.
Two hitherto unknown bacteria (strains 313 and 352) were recovered from the faeces of Tibetan antelopes on the Tibet-Qinghai Plateau, PR China. Cells were rod-shaped and Gram-stain-positive. The optimal growth conditions were at 37 °C and pH 7. The isolates were closely related to (92.6 % 16S rRNA gene sequence similarity), (92.5 %), (92.4 %), (92.2 %) and (91.6 %). Phylogenetic analyses showed that strains 313 and 352 clustered independently in the vicinity of the genera , and , but could not be classified clearly as a member of any of these genera. Phylogenomic analysis also indicated that strains 313 and 352 formed an independent branch in the family . The major cellular fatty acids of the strains were C and Cω9. The polar lipids comprised diphosphatidylglycerol, phosphatidylinositol mannoside, phosphatidylglycerol, phosphatidylinositol and five unidentified components. The peptidoglycan contained lysine, alanine and glutamic acid. The respiratory quinone was absent. The whole-cell sugars included glucose and rhamnose. The DNA G+C content of strain 313 was 60.6 mol%. Based on the low 16S rRNA gene sequence similarities, its taxonomic position in the phylogenetic and phylogenomic trees and its unique lipid pattern, we propose that strains 313 and 352 represent members of a novel species in a new genus, for which the name gen. nov., sp. nov. is proposed. The type strain is 313 (=CGMCC 4.7453=DSM 106216).
从中国青藏高原藏羚羊粪便中分离出两株此前未知的细菌（菌株313和352）。细胞呈杆状，革兰氏染色阳性。最佳生长条件为37 °C和pH 7。这些分离株与 （16S rRNA基因序列相似性为92.6 %）、 （92.5 %）、 （92.4 %）、 （92.2 %）和 （91.6 %）密切相关。系统发育分析表明，菌株313和352在 、 和 属附近独立聚类，但不能明确归类为这些属中的任何一个成员。系统基因组分析还表明，菌株313和352在该科中形成一个独立分支。这些菌株的主要细胞脂肪酸为C 和Cω9。极性脂质包括双磷脂酰甘油、磷脂酰肌醇甘露糖苷、磷脂酰甘油、磷脂酰肌醇和五种未鉴定成分。肽聚糖含有赖氨酸、丙氨酸和谷氨酸。呼吸醌不存在。全细胞糖包括葡萄糖和鼠李糖。菌株313的DNA G+C含量为60.6 mol%。基于低16S rRNA基因序列相似性、其在系统发育树和系统基因组树中的分类位置以及其独特的脂质模式，我们提议菌株313和352代表一个新属中的一个新物种的成员，为此提出新属名 ，新种名 。模式菌株为313（=CGMCC 4.7453=DSM 106216）。
