Opt Lett. 2019 Nov 1;44(21):5238-5241. doi: 10.1364/OL.44.005238.
We demonstrate dual-Airy-beam-scanning-based volumetric two-photon microscopy (TPM) with depth-resolving capability. A pair of Airy beams with opposite acceleration is used as the excitation lights to sequentially illuminate the sample, and depth information can be resolved based on the deflection of the Airy beam. The depth-resolving range of the volumetric TPM is up to 32 μm. The advantages of the depth-resolved volumetric TPM are the depth-resolving capability over Bessel-beam-based TPM and less scanning times over traditional Gaussian-beam-based TPM. The depth-resolved volumetric TPM provides a promising fast imaging tool to study the dynamics in neural biology.
我们展示了基于双艾里光束扫描的具有深度分辨能力的体积双光子显微镜（TPM）。一对具有相反加速度的艾里光束被用作激发光，依次照射样品，并且可以基于艾里光束的偏折来分辨深度信息。体积 TPM 的深度分辨范围可达 32 μm。深度分辨体积 TPM 的优点是相对于贝塞尔光束 TPM 具有深度分辨能力，并且相对于传统的高斯光束 TPM 具有更少的扫描次数。深度分辨体积 TPM 为研究神经生物学中的动力学提供了一种有前途的快速成像工具。
