Földes O, Langer P, Brozmanová H, Gschwendtová K
Endocrinol Exp. 1979;13(2):105-10.
In three groups of male rats weighing about 350 g and fed normal pelleted diet the left femoral artery and vein were cannulated under pentobarbiturate anesthesia and in samples of plasma obtained between 0 and 6 h after the injection of heparin the level of thyroxine (T4) was measured with the aid of specific radioimmunoassay. In addition, in Group A (22 animals) and C (17 animals) also the bile duct was cannulated and the total bile was collected into a flask for the same period. In addition, the animals of a Group C were thyroidectomized by electrocoagulation immediately before "O h". The Group B consisted of 18 sham-operated animals in which, in addition to the cannulation of blood vessels, only laparotomy and separation of bile duct without cannulation were made. In all groups a linear decline of plasma T4 level was found corresponding to a half-life of about 11 h. Since there were no significant differences in the slope of regression line between groups, it was concluded that the period of draining bile duct for 6 h was presumably too short to interfere significantly with the absorption of T4 in lower intestine and ileum and that the uniform decline of T4 level in plasma in all groups was presumably due to the effect of pentobarbiturate.
在三组体重约350克、喂食普通颗粒饲料的雄性大鼠中，在戊巴比妥麻醉下对左股动脉和静脉进行插管，并在注射肝素后0至6小时采集的血浆样本中，借助特异性放射免疫测定法测量甲状腺素（T4）水平。此外，在A组（22只动物）和C组（17只动物）中，还对胆管进行插管，并在同一时期将总胆汁收集到一个烧瓶中。另外，C组的动物在“0小时”前立即通过电凝法进行甲状腺切除。B组由18只假手术动物组成，除了血管插管外，只进行了剖腹术和胆管分离但未插管。在所有组中均发现血浆T4水平呈线性下降，半衰期约为11小时。由于各组之间回归线斜率无显著差异，因此得出结论，胆管引流6小时的时间可能太短，无法显著干扰T4在小肠下段和回肠的吸收，且所有组血浆中T4水平的均匀下降可能是由于戊巴比妥的作用。
