Földes O, Langer P, Brozmanová H, Gschwendtová K
Endocrinol Exp. 1980 Sep;14(3):215-20.
Male Wistar rats weighing about 400 g were anaesthetized with pentobarbiturate (40 mg kg-1) i.p. and thin polyethylene tubings were inserted in left femoral artery and vein as well as in a bile duct. Blood samples were taken at 30-60 min intervals for 6 h. The total bile was collected to pre-weighed glass vials. With the aid of radioimmunoassay the level of thyroxine (T4) and 3,5,3'-triiodothyronine (T3) was estimated in plasma and the total excretion of these compounds by bile was measured after the incubation of bile aliquots with beta-glucuronidase-arylsulphatase. After i.v. injection of sodium salicylate (S; 80 mg kg-1) a significant decrease of T4 and T3 in plasma was found compared either to the initial level found in the same animals or to that found in controls not injected with S. Total excretion of bile was increased within 2 h after the administration of S. Under these conditions, the excretion of T4 was increased, while that of T3 was decreased. It is suggested that the effect of salicylate under the experimental conditions used consists of: 1. displacement of iodothyronines from plasma protein binding; 2. increased bile excretion (during the initial period after the administration of S) presumably due to the increased blood flow through the liver.
体重约400克的雄性Wistar大鼠经腹腔注射戊巴比妥钠（40毫克/千克）麻醉，将细聚乙烯管插入左股动脉、静脉以及胆管。每隔30 - 60分钟采集一次血样，共采集6小时。将总胆汁收集到预先称重的玻璃瓶中。借助放射免疫分析法测定血浆中甲状腺素（T4）和3,5,3'-三碘甲状腺原氨酸（T3）的水平，并在胆汁等分试样与β-葡萄糖醛酸酶 - 芳基硫酸酯酶孵育后测量这些化合物经胆汁的总排泄量。静脉注射水杨酸钠（S；80毫克/千克）后，与同一动物的初始水平或未注射S的对照组相比，血浆中T4和T3显著降低。给药后2小时内胆汁总排泄量增加。在这些条件下，T4的排泄增加，而T3的排泄减少。提示在所使用的实验条件下，水杨酸盐的作用包括：1. 从血浆蛋白结合中置换出碘甲状腺原氨酸；2. 胆汁排泄增加（在给药后的初始阶段），可能是由于流经肝脏的血流量增加。
