Reinke R, Zipursky S L
Department of Biological Chemistry, UCLA School of Medicine.
Cell. 1988 Oct 21;55(2):321-30. doi: 10.1016/0092-8674(88)90055-4.
Genetic mosaic studies indicate that environmental cues play a critical role in photoreceptor cell (R-cell) development in the Drosophila compound eye. Recent analysis of the sevenless gene suggests that its product, a cell surface protein containing a putative intracellular domain homologous to tryosine kinases, is a receptor for a signal specifying an R7-specific pathway of cellular development. We report the identification of a second genetic locus, bride of sevenless (boss) required for the development of R7. Genetic mosaic analysis demonstrates that boss expression is required in the R8 cell for a neighboring cell to become R7. However, the development of R1-R6 and R8 is boss-independent. These data indicate a central role for R8 in directing ommatidial assembly and are consistent with boss encoding the ligand recognized by the sevenless receptor.
基因镶嵌研究表明，环境线索在果蝇复眼中光感受器细胞（R细胞）的发育过程中起着关键作用。最近对无七基因的分析表明，其产物是一种细胞表面蛋白，含有一个与酪氨酸激酶同源的假定细胞内结构域，是一种信号受体，该信号指定了细胞发育的R7特异性途径。我们报告了另一个R7发育所需的基因位点——无七之妻（boss）的鉴定。基因镶嵌分析表明，R8细胞中需要有boss表达，相邻细胞才能发育为R7。然而，R1-R6和R8的发育不依赖于boss。这些数据表明R8在指导小眼装配中起核心作用，并且与boss编码无七受体所识别的配体这一观点一致。
