Chang Yanxiang, Yang Lisheng
Department of Cardiothoracic Surgery, The First Affiliated Hospital of Xi'an Medical University, Xi'an, Shaanxi, China.
Department of Pulmonary Disease, Xi'an Traditional Chinese Medicine Hospital, Xi'an, Shaanxi, China.
J Cell Biochem. 2020 Aug;121(8-9):3691-3699. doi: 10.1002/jcb.29510. Epub 2019 Nov 3.
Lung adenocarcinoma (LAD), as one of the most common types of lung tumors, is lethal and malignant. Long noncoding RNAs (lncRNAs) play important roles in various cancers according to many previous studies. LINC00467 was proposed to be a tumor promoter. Despite the validated promotive effect of LINC00467 on neuroblastoma progression, its regulatory mechanism in LAD remains unclear. In this study, LINC00467 expressed higher in LAD tissues and cell lines, and increased LINC00467 indicated a poor prognosis. Knockdown of LINC00467 inhibited cell proliferation, the expressions of tumor stem cell-related genes, and cell spheroid formation ability, while it promoted cell apoptosis. miR-4779 and miR-7978 were reported to play antitumor roles in several cancers before. LINC00467 could combine with miR-4779 and miR-7978, and negatively regulated miR-4779 and miR-7978. miR-4779 and miR-7978 inhibitor could partly rescue the LINC00467 knockdown-induced influence on cell proliferation, apoptosis, and stemness. In a word, this study innovatively investigated the mechanism of LINC00467 in LAD and verified LINC00467 exerted its carcinogenesis function by sponging miR-4779 and miR-7978, which may become a catalyst for generating new therapeutic targets for LAD treatment.
肺腺癌（LAD）作为最常见的肺肿瘤类型之一，具有致死性和恶性。根据以往许多研究，长链非编码RNA（lncRNAs）在各种癌症中发挥着重要作用。LINC00467被认为是一种肿瘤促进因子。尽管已证实LINC00467对神经母细胞瘤进展具有促进作用，但其在LAD中的调控机制仍不清楚。在本研究中，LINC00467在LAD组织和细胞系中表达较高，LINC00467升高表明预后不良。敲低LINC00467可抑制细胞增殖、肿瘤干细胞相关基因的表达以及细胞球形成能力，同时促进细胞凋亡。据报道，miR-4779和miR-7978在几种癌症中发挥抗肿瘤作用。LINC00467可与miR-4779和miR-7978结合，并对miR-4779和miR-7978起负调控作用。miR-4779和miR-7978抑制剂可部分挽救LINC00467敲低对细胞增殖、凋亡和干性的影响。总之，本研究创新性地探讨了LINC00467在LAD中的作用机制，并证实LINC00467通过海绵吸附miR-4779和miR-7978发挥其致癌功能，这可能成为为LAD治疗产生新治疗靶点的催化剂。
