Lu Mingdian, Liu Dong, Li Yongxiang
Department of Gastrointestinal Surgery and General Surgery, First Affiliated Hospital of Anhui Medical University, Hefei, 230022, Anhui, People's Republic of China.
Cell Death Discov. 2022 Mar 5;8(1):100. doi: 10.1038/s41420-022-00875-z.
Emerging evidence indicated that long noncoding RNAs (lncRNAs) and microRNAs (miRNAs) exert critical effects on tumorigenesis of multiple malignancies, including gastric cancer (GC). We aim to explore the effects of long intergenic non-protein coding RNA 467 (LINC00467) and miR-27b-3p on GC. GC cells were initially cultured. LINC00467, miR-27b-3p, and signal transducer and activator of transcription 3 (STAT3) expression in GC were detected. The altered LINC00467 and/or miR-27b-3p were transfected into screened cells. Then, the biological activities of GC cells and the tumor growth in vivo were examined. The binding relationships among LINC00467, miR-27b-3p, and STAT3 were confirmed. It was indicated that LINC00467 was increased while miR-27b-3p was decreased in GC tissues and cells. Inhibition of LINC00467 hindered GC cell malignancy and blocked tumor development by upregulating miR-27b-3p. LINC00467 sponged miR-27b-3p and STAT3 was targeted by miR-27b-3p. It was discovered that LINC00467 reduction upregulates miR-27b-3p to repress malignant GC cell growth via inhibiting STAT3. This research may deepen the insight of molecular mechanisms on GC.
新出现的证据表明，长链非编码RNA（lncRNAs）和微小RNA（miRNAs）对包括胃癌（GC）在内的多种恶性肿瘤的发生发展具有关键作用。我们旨在探讨长链基因间非编码RNA 467（LINC00467）和miR-27b-3p对胃癌的影响。首先培养胃癌细胞。检测胃癌中LINC00467、miR-27b-3p和信号转导及转录激活因子3（STAT3）的表达。将改变后的LINC00467和/或miR-27b-3p转染到筛选出的细胞中。然后，检测胃癌细胞的生物学活性和体内肿瘤生长情况。证实了LINC00467、miR-27b-3p和STAT3之间的结合关系。结果表明，在胃癌组织和细胞中LINC00467表达增加而miR-27b-3p表达降低。抑制LINC00467可通过上调miR-27b-3p来阻碍胃癌细胞的恶性程度并阻断肿瘤发展。LINC00467可吸附miR-27b-3p，且STAT3是miR-27b-3p的靶标。研究发现，降低LINC00467可上调miR-27b-3p，通过抑制STAT3来抑制胃癌细胞的恶性生长。本研究可能会加深对胃癌分子机制的认识。
