Yue Yang, Ma Chao, Zhang Yan, Chen Hong-Song, Guo Jian-Ying, Liu Ting-Hui, Zhou Zhong-Shi
College of Plant Protection, Hebei Agricultural University, Baoding 071001, China.
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
Insects. 2023 Feb 14;14(2):190. doi: 10.3390/insects14020190.
The olfactory system plays a key role in various insect behaviors, and odorant-binding proteins participate in the first step of the olfactory process. Lesage is an oligophagous phytophagous insect that is a specific biological control agent for L. The leaf beetle must identify and locate through olfaction; however, its odorant-binding protein (OBP) function has not yet been reported. In this study, was cloned, and its tissue expression profile and binding ability were analyzed using RT-qPCR and fluorescence binding assays, respectively. Sequence analysis demonstrated that belongs to the classical OBP family. The RT-qPCR results showed that was specifically expressed in the antennae, indicating that may be involved in chemical communication. The fluorescence binding assay showed that has an extensive binding ability to alkenes. The electroantennography experiments showed that antennal response to α-pinene and ocimene decreased significantly after interference because the two odors specifically bound to . In summary, α-pinene and ocimene are odorant ligands corresponding to , indicating that is involved in the chemical recognition of Our study lays a theoretical foundation for research into attractants, which is helpful for the better biological control of by .
嗅觉系统在多种昆虫行为中起着关键作用，气味结合蛋白参与嗅觉过程的第一步。牧草盲蝽是一种寡食性植食性昆虫，是L的一种特定生物防治剂。叶甲必须通过嗅觉识别和定位；然而，其气味结合蛋白（OBP）的功能尚未见报道。在本研究中，对其进行了克隆，并分别使用RT-qPCR和荧光结合试验分析了其组织表达谱和结合能力。序列分析表明，其属于经典的OBP家族。RT-qPCR结果表明，其在触角中特异性表达，表明其可能参与化学通讯。荧光结合试验表明，其对烯烃具有广泛的结合能力。触角电位实验表明，干扰后其对α-蒎烯和罗勒烯的触角反应显著降低，因为这两种气味与该蛋白特异性结合。综上所述，α-蒎烯和罗勒烯是该蛋白对应的气味配体，表明该蛋白参与牧草盲蝽的化学识别。我们的研究为牧草盲蝽引诱剂的研究奠定了理论基础，有助于更好地利用牧草盲蝽对L进行生物防治。
