Shearer J D, Amaral J F, Caldwell M D
Department of Surgery, Brown University, Providence, RI.
Circ Shock. 1988 Jul;25(3):131-8.
Wounded muscle which has increased glucose uptake is infiltrated with a large number of inflammatory cells. Macrophages which are the predominant inflammatory cells in wounded muscle can increase glucose oxidation by epididymal fat pads. Therefore, the possibility that inflammatory cells could also alter glucose metabolism in skeletal muscle was investigated. Peritoneal inflammatory cells (67% macrophages, 7% lymphocytes, 15% polymorphonuclear leukocytes, and 11% eosinophils) were elicited with a 1% sodium caseinate solution. Extensor digitorum longus muscles, were incubated alone, in the presence of inflammatory cells or in an inflammatory-cell-conditioned media. Similar incubations were performed using epididymal fat pads. In addition, inflammatory cells were incubated alone. The present studies confirmed the previous finding that inflammatory cells release a factor(s) that increases glucose oxidation in epididymal fat pads. The coincubation of inflammatory cells with skeletal muscle resulted in a 22% increase in glucose uptake and a 19% increase in the conversion of glucose to lactate compared to the sum of these processes for muscle or inflammatory cells incubated alone. In addition, this study showed that the incubation of skeletal muscle in inflammatory-cell-conditioned medium produced a 117.6% increase in glucose uptake and a 147% increase in the conversion of glucose to lactate. Therefore, inflammatory cells can increase glucose metabolism in skeletal muscle through the release of a soluble factor(s). This effect does not require a direct contact of the inflammatory cells with skeletal muscle.
葡萄糖摄取增加的受伤肌肉中浸润着大量炎性细胞。巨噬细胞是受伤肌肉中主要的炎性细胞，其可通过附睾脂肪垫增加葡萄糖氧化。因此，研究了炎性细胞是否也能改变骨骼肌中的葡萄糖代谢。用1%酪蛋白酸钠溶液诱导产生腹膜炎性细胞（67%为巨噬细胞、7%为淋巴细胞、15%为多形核白细胞、11%为嗜酸性粒细胞）。将趾长伸肌单独孵育，或在炎性细胞存在的情况下孵育，或在炎性细胞条件培养基中孵育。使用附睾脂肪垫进行类似的孵育。此外，将炎性细胞单独孵育。本研究证实了先前的发现，即炎性细胞释放一种或多种可增加附睾脂肪垫中葡萄糖氧化的因子。与单独孵育的肌肉或炎性细胞相比，炎性细胞与骨骼肌共同孵育导致葡萄糖摄取增加22%，葡萄糖向乳酸的转化增加19%。此外，本研究表明，骨骼肌在炎性细胞条件培养基中孵育导致葡萄糖摄取增加117.6%，葡萄糖向乳酸的转化增加147%。因此，炎性细胞可通过释放一种或多种可溶性因子增加骨骼肌中的葡萄糖代谢。这种作用不需要炎性细胞与骨骼肌直接接触。
